{"product_id":"biolegend-330506","title":"Biolegend, 330506, PE anti-human TCL1 Antibody, 100tests","description":"\u003cp\u003eTCL1, also known as T-cell leukemia\/lymphoma protein 1A, protein p14 TCL1 and oncogene TCL-1, is a 14kD beta-barrel protein that was originally identified on the basis of its association with T-cell prolymphocytic leukemia (T-PLL). Activated by the T-cell receptor enhancer through inv(14)(q11;q32.1) or t(14;14)(q11;q32.1), TCL1 is normally expressed in immature cortical thymocytes, activated peripheral T-cells,  pro-B cells, and naive mantle zone B cells of peripheral lymphoid tissues, but not in post-germinal center (GC) memory B cells and plasma cells. Akt and TCL1 interact via Akt’s pleckstrin homology domain to form hetero-oligomers at the leaflet of the inner plasma membrane stimulating the protein kinase C-mitogen activated protein kinase-extracellular signal-related kinase (PKC-MAPK-ERK) signal transduction pathway. TCL1 also inhibits activation-induced cell death in T-cells by blocking PKCθ. In addition to T-PLL dysregulation is implicated in such B-cell neoplasias as lymphoblastic lymphoma, chronic lymphocytic leukemia (CLL) and Burkitt lymphoma.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: outer α-loop region of TCL1 peptide\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications include: immunoprecipitation, western blot, immunohistochemical staining of frozen or formalin-fixed, paraffin embedded tissue sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Herling M, et al. 2008. Blood 111:328 Herling M, et al. 2007. Am. J. Surg. Pathol. 31:1123\u003cbr\u003e\nProduct Citations: O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed\u003cbr\u003e\nRRID: AB_2204407 (BioLegend Cat. No. 330506)\u003cbr\u003e\nStructure: Beta barrel protein, 14kD\u003cbr\u003e\nDistribution: Cytoplasm\u003cbr\u003e\nFunction: Enhances Akt mediated cell survival, stabilizes mitochondrial membrane potential\u003cbr\u003e\nInteraction: Akt1, Akt2, Akt3, caco2\u003cbr\u003e\nBiology Area: Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Narducci MG et al. 2000. Cancer Research 60:2095. 2. Teitell MA et al. 2005 Nat. Rev. Cancer 5:640 3. Laine J et al.2000. Molecular Cell 6:395 4. Virgilio L et al. 1994. Proc. Natl. Acad. Sci. 91:12530 5. Masayuk N et al. 2007 FASEB J. 21:2273 6. Despouy G et al. 2007 Blood 110:4406 7. Rhine S et al. 2006 Blood 108:1991\u003cbr\u003e\nGene ID: 8115\u003cbr\u003e\nUniProt: View information about TCL1 on UniProt.org\u003cbr\u003e\nClone: 1-21\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T-cell leukemia\/lymphoma protein 1A, Protein p14 TCL1, Oncogene TCL1, TCL-1\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860766740649,"sku":"330506","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-330506","provider":"Iright","version":"1.0","type":"link"}