{"product_id":"biolegend-331116","title":"Biolegend, 331116, PE\/Cyanine7 anti-human\/mouse CXCR7 Antibody, 100tests","description":"\u003cp\u003eCXCR7, also known as RDC1, belongs to a subgroup of C-X-C chemokine receptors, which are part of a large protein family of G protein-coupled receptors (GPCR). CXCR7 binds with high-affinity to CXCL12\/SDF-1 and CXCL11\/I-TAC, which regulates the trafficking and activation of leukocytes. It is also a co-receptor for the entry of HIV-1. Binding of ligand to CXCR7 induces proliferation and migration of immature neurons, glia and their precursors. CXCR7 expression occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. Surface expression of CXCR7 was also reported to be on tumor cells, activated endothelial cells, fetal liver cells, and other cell types.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human CXCR7 transfectant\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Soehnlein O, et al. 2013. EMBO Mol. Med. 5:471. (FC) PubMed Balabanian K, et al. 2012. J. Transl. Med. 10:251. (FC) PubMed\u003cbr\u003e\nProduct Citations: Ceholski D, et al. 2017. Stem Cell Res. 10.1016\/j.scr.2017.06.015. PubMed\u003cbr\u003e\nRRID: AB_2565678 (BioLegend Cat. No. 331115) AB_2565679 (BioLegend Cat. No. 331116)\u003cbr\u003e\nStructure: G protein-coupled receptor super family\u003cbr\u003e\nDistribution: Monocytes, B cells, T cells and mature dendritic cells\u003cbr\u003e\nLigand\/Receptor: CXCL12\/SDF-1 and CXCL11\/I-TAC\u003cbr\u003e\nCell Sources: On a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Monocytes, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Burns JM, et al. 2006. J. Exp. Med. 203:2201. 2. Infantino S, et al. 2006. J. Immunol. 176:2197.\u003cbr\u003e\nRegulation: Regulates the trafficking and activation of leukocytes\u003cbr\u003e\nGene ID: 1277857007\u003cbr\u003e\nUniProt: View information about CXCR7 on UniProt.org\u003cbr\u003e\nClone: 8F11-M16\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Chemokine (C-X-C motif) receptor 7, RDC1, CMKOR1, GPR159\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862495219881,"sku":"331116","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-331116","provider":"Iright","version":"1.0","type":"link"}