{"product_id":"biolegend-331606","title":"Biolegend, 331606, PE anti-human CD276 (B7-H3) Antibody, 100tests","description":"\u003cp\u003eB7-H3 is a member of the B7 gene family. It has 88% amino acid sequence identity with mouse B7-H3. It is mostly expressed on professional APCs, including B cells, macrophages, and dendritic cells. Its expression on dendritic cells appears to be up-regulated by LPS. B7-H3 protein inhibits T cell activation and cytokine production. It was reported that monoclonal antibody against B7-H3 enhanced T cell proliferation in vitro and led to exacerbated EAE in vivo. Therefore, B7-H3 may be a negative regulator on T cell activation and function.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nProduct Citations: Watanabe T, et al. 2023. Polymers (Basel). 15:. PubMed Shao L, et al. 2021. Cell Death Discov. 7:145. PubMed Fang C, et al. 2022. J Pathol. 256:310. PubMed Zeng W, et al. 2017. Front Immunol. 0.806944444. PubMed Brignole C, et al. 2021. J Exp Clin Cancer Res. 40:180. PubMed Huang C, et al. 2020. Theranostics. 10:10498. PubMed Liu T, et al. 2022. Front Immunol. 13:901349. PubMed Xie J, et al. 2021. FEBS Open Bio. 11:2977. PubMed Lavoie RR, et al. 2021. Cancers (Basel). 13:. PubMed Li H, et al. 2020. Front Oncol. 1.477083333. PubMed\u003cbr\u003e\nRRID: AB_1279200 (BioLegend Cat. No. 331605) AB_1279197 (BioLegend Cat. No. 331606)\u003cbr\u003e\nStructure: About 49.7 KD transmembrane protein characterized by extracellular IgV and IgC domains.\u003cbr\u003e\nDistribution: B7-H3 is expressed on professional APCs, including B cells, dendritic cells, and macrophages. It is reported to express on a minor fraction of CD4+ and CD8+ cells.\u003cbr\u003e\nLigand\/Receptor: The receptor has not been identified, but it seems to be expressed on activated T cells.\u003cbr\u003e\nBioactivity: B7-H3 is a negative regulator of T cell activation and IL-2 production.\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Sun M, et al. 2002. J. Immunology. 168:6294 2. Xu J, et al. 2006. Cellular and Molecular Immunology. 3:235\u003cbr\u003e\nGene ID: 80381\u003cbr\u003e\nUniProt: View information about CD276 on UniProt.org\u003cbr\u003e\nClone: DCN.70\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B7RP2\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863005679785,"sku":"331606","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-331606","provider":"Iright","version":"1.0","type":"link"}