{"product_id":"biolegend-331914","title":"Biolegend, 331914, Brilliant Violet 421™ anti-human CD335 (NKp46) Antibody, 100tests","description":"\u003cp\u003eCD335, also known as NKp46, is a member of the natural cytotoxicity receptor (NCR) family which triggers cytotoxicity in NK cells. CD335 is direct­ly involved in target cell recognition and lysis, and is exclusively expressed on CD3 - CD56 + NK cells, suggesting it is a universal marker for NK cells. NKp46, along with NKp30 and NKp44, is referred to as a natural cytoxicity receptor (NCR) and plays a very important role in killing virus-infected tumor cells and MHC-class I-unprotected cells.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: NKp46-Fc fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Clone 9E2 has been shown to block NK activation through NKp46.6\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309. Kalberer CP, et al. 2003. Blood 102:127. Chen Y, et al. 2007. J. Immunol. 179:2766. Jarahian M, et al. 2009. J. Virol. 83:8108. PubMed Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Achdout H. et al. 2010. J. Virol. 84:3993.\u003cbr\u003e\nProduct Citations: Lukas H, et al. 2023. National Library of Medicine. . 120(33): e2300343120. PubMed Apte SH, et al. 2020. Clin Transl Immunology. 9:e1209. PubMed Heger L, et al. 2018. Front Immunol. 9:744. PubMed Maas RJ, et al. 2020. Oncoimmunology. 9:1843247. PubMed Mishra HK, et al. 2021. Front Immunol. 12:711621. PubMed Alonso R, et al. 2022. Blood Adv. :. PubMed Antonarakis ES, et al. 2018. Clin Cancer Res. 24:4662. PubMed Sagebiel AF, et al. 2019. Nat Commun. 10:975. PubMed Lee J, et al. 2015. J Exp Med. 212:385. PubMed Marquardt N, et al. 2015. J Immunol. 194:2467. PubMed Baguma R, et al. 2017. PLoS One. 10.1371\/journal.pone.0184563. PubMed\u003cbr\u003e\nRRID: AB_10916529 (BioLegend Cat. No. 331913) AB_2563853 (BioLegend Cat. No. 331914)\u003cbr\u003e\nStructure: Type I membrane glycoprotein (46 kD)\u003cbr\u003e\nDistribution: Expressed on resting and activated NK cells\u003cbr\u003e\nCell Type: NK cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147. 2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309. 3. Sivori S. 1999. Eur. J. Immunol. 29:1656.\u003cbr\u003e\nGene ID: 9437\u003cbr\u003e\nUniProt: View information about CD335 on UniProt.org\u003cbr\u003e\nClone: 9E2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: NKp46, NCR1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862509015209,"sku":"331914","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-331914","provider":"Iright","version":"1.0","type":"link"}