{"product_id":"biolegend-333401","title":"Biolegend, 333401, Purified anti-human CD35 Antibody, 25μg","description":"\u003cp\u003eCD35 is a type I single chain of glycoprotein, also known as C3b\/C4b receptor, Complement Receptor type 1 or CR1. Four molecular weight allotypes (160kD, 190kD, 220kD, and 250kD) have been described. CD35 is expressed on granulocytes, monocytes, B cells, erythrocytes, and follicular dendritic cells, as well as subsets of NK and T cells. CD35 binds complement C3b, C4b, or iC3, and iC4, and plays important roles in both innate and adoptive immune response via mediating phagocytosis by granulocytes and monocytes. CD35 has also been reported to inhibit T-cell proliferation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IP, IHC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hogg N, et al. 1984. Eur. J. Immunol. 14:236 Zabeleta J, et al. 1998. Clin Diag. Lab. Immunol. 5:690 Pascual M, et. al. 1994. Eur. J. Immunol. 24:702 Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Weinhage T, et al. 2020. J Immunol. 205:56. PubMed Syrimi E, et al. 2021. iScience. 24:103215. PubMed Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed\u003cbr\u003e\nRRID: AB_1089032 (BioLegend Cat. No. 333402)\u003cbr\u003e\nStructure: Type I glycoprotein with four allotypes, 160kD, 190kD, 220kD, 250kD\u003cbr\u003e\nDistribution: Granulocytes, monocytes, B cells, erythrocytes, follicular dendritic cells, subsets of NK and T cells\u003cbr\u003e\nFunction: Adhesion, mediate phagocytosis, inhibit T-cell proliferation\u003cbr\u003e\nLigand\/Receptor: C3b, iC3, C4b, iC4\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Erythrocytes, Granulocytes, Monocytes, NK cells, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Complement, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Zola Heddy, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD markers. WILEY-LISS 2. Klickstein LB, et al. 1988. J. Exp. Med. 168:1699\u003cbr\u003e\nGene ID: 1378\u003cbr\u003e\nUniProt: View information about CD35 on UniProt.org\u003cbr\u003e\nClone: E11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: III 204\u003cbr\u003e\nOther Names: C3b\/C4b receptor, complement receptor type 1 (CR1)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863064006825,"sku":"333401","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-333401","provider":"Iright","version":"1.0","type":"link"}