{"product_id":"biolegend-334203","title":"Biolegend, 334203, Biotin anti-human CD202b (Tie2\/Tek) Antibody, 25μg","description":"\u003cp\u003eCD202b is a 145 kD type I transmembrane protein, also known as Tie2 or TEK. It is a member of the receptor tyrosine kinase (RTK) family of proteins and is expressed by endothelial cells and their progenitors, quiescent hematopoietic stem cells (HSCs), and a subpopulation of monocytes. Angiopoietin-1 (Ang-1) is an activator of CD202b to promote, maintain, and stabilize mature vessels and to maintain HSCs in quiescent state. Ang-2 is another ligand of CD202b, which is involved in postnatal angiogenesis and in antagonizing the effects of Ang-1. Tie2 binds to Ang-4 as well.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant extracellular domain of human Tie2\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications include: immunoprecipitation, Wstern blot, immunohistochemical staining of frozen tissue sections and ELISA\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Peters KG, et al. 1998. Br. J. Cancer. 77:51. Wong AL, et al. 1997. Circ. Res. 81:567. Lin P, et al. 1998. P. Natl. Acad. Sci. USA 95:8829.\u003cbr\u003e\nProduct Citations: Hoover MY, et al. 2023. Nat Protoc. . PubMed Tevlin R, et al. 2023. Sci Rep. 13:2097. PubMed Goodnough LH, et al. 2020. JBMR Plus. 4:e10398. PubMed Ambrosi TH, et al. 2020. Aging Cell. 19:e13164. PubMed\u003cbr\u003e\nRRID: AB_1186046 (BioLegend Cat. No. 334204)\u003cbr\u003e\nStructure: 145 kD type I transmembrane protein, receptor tyrosine kinase (RTK) family\u003cbr\u003e\nDistribution: Endothelial cells, subsets of hematopoietic stem cells and and a subset of monocytes\u003cbr\u003e\nFunction: Angiogenesis, hemotopoiesis, monocytes chemotaxis\u003cbr\u003e\nLigand\/Receptor: Angiopoietin-1 (Ang-1), Ang-2, and Ang-4\u003cbr\u003e\nCell Type: Endothelial cells, Hematopoietic stem and progenitors, Monocytes\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1.De Palma M, et al. 2005. Cancer Cell. 8:211 2.Shaw JP, et al. 2004. Blood Cells Mol. Dis. 32:168 3. Hsu HC, et al. 2000. Blood. 96:3757 4. Arai F, et al. 2004. Cell. 118:149\u003cbr\u003e\nGene ID: 7010\u003cbr\u003e\nUniProt: View information about CD202b on UniProt.org\u003cbr\u003e\nClone: 33.1 (Ab33)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Tie2, TEK\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862370996393,"sku":"334203","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-334203","provider":"Iright","version":"1.0","type":"link"}