{"product_id":"biolegend-340203","title":"Biolegend, 340203, PE anti-human CD307d (FcRL4) Antibody, 25tests","description":"\u003cp\u003eFcRL4 (Fc receptor-like 4) is a 57 kD single pass type I transmembrane protein that exhibits 4 Ig like C2 domains. Also known as ITRA1, IGFP2, and FcRH4, FcRL4 is predominately found on a subset of memory B cells. Activation of FcRL4 contributes to B cell receptor inhibition through activation of the tyrosine kinases SHP-1 and 2. FcRL4's nascent loci is 1q23.1; however, breakpoint mutations are associated with multiple myeloma and non-Hodgkins lymphoma. Increased FcRL4 expression on memory B cells has also been observed in HIV viremic patients. FcRL4 predominately binds aggregated IgA, binds weakly with IgG3 and IgG4, and does not bind IgG1 and IgG2.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: P815 cells transfected with FcRL4\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (with relevant formats) include: immunoprecipitation and blocking. mAb 413D12 is able to block the binding of FcRL4 to aggregated IgA, IgG3, and IgG4.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Sohn HW, et al. 2011. Blood. 118:6332. PubMed Wilson TJ, et al. 2012. J. Immunol. 188:4741. (Block)\u003cbr\u003e\nProduct Citations: Oliviero B, et al. 2011. J Hepatol. 55:53. PubMed SoRelle ED, et al. 2022. Front Immunol. 13:1001145. PubMed Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed Parmigiani A, et al. 2013. PLoS One. 13:79816. PubMed Sohn H, et al. 2011. Blood. 118:6332. PubMed Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed Amara K, et al. 2017. J Autoimmun. 10.1016\/j.jaut.2017.03.004. PubMed Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed Jenks SA et al. 2018. Immunity. 49(4):725-739 . PubMed Upasani V, et al. 2019. Front Immunol. 2.152777778. PubMed\u003cbr\u003e\nRRID: AB_1575103 (BioLegend Cat. No. 340203) AB_2104578 (BioLegend Cat. No. 340204)\u003cbr\u003e\nStructure: 57 kD, Fc receptor like, four Ig like domains\u003cbr\u003e\nDistribution: Subset of memory B cells, some multiple myeloma and non-Hodgkins lymphoma\u003cbr\u003e\nInteraction: Tyrosine phophorylated FcRL4 interacts with SHP-1, SHP-2 to inhibit the B cell receptor\u003cbr\u003e\nBioactivity: B-cell receptor inhibition\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Fc Receptors\u003cbr\u003e\nAntigen References: 1. Moir S, et al. 2008. J. Exp. Med. 205:1797. 2. Ehrhardt GRA, et al. 2003. P. Natl. Acad. Sci. USA 100:13489. 3. Ehrhardt GRA, et al. 2005. J. Exp. Med. 202:783. 4. Polson AG, et al. 2006. J. Int. Immunol. 18:1363.\u003cbr\u003e\nGene ID: 83417\u003cbr\u003e\nUniProt: View information about CD307d on UniProt.org\u003cbr\u003e\nClone: 413D12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: FcRL4 (Fc receptor-like 4), IRTA1, IGFP2\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862400880809,"sku":"340203","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-340203","provider":"Iright","version":"1.0","type":"link"}