{"product_id":"biolegend-342611","title":"Biolegend, 342611, Brilliant Violet 421™ anti-human HLA-E Antibody, 25tests","description":"\u003cp\u003eHLA-E is a non-classical MHC class I (MHC-Ib) molecule. It is characterized by limited polymorphism and ubiquitous expression. HLA-E, as other MHC-I molecules, is heterodimerized with β2-microglobulin. HLA-E interacts with CD94\/NKG2 receptor to regulate NK cell cytolysis activities.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Lee N, et al. 1998. Proc. Natl. Acad. Sci. USA. 95:5199. Wooden SL, et al. 2005. J. Immunol. 175:1383. Monaco EL, et al. 2008. J. Immunol. 181:5442. Corrah TW, et al. 2011. J. Virol. 85:3367. PubMed\u003cbr\u003e\nProduct Citations: Sun W, et al. 2023. Nature. 614:309. PubMed Zhuang X, et al. 2020. Front Immunol. 2.415972222. PubMed van Stigt Thans T, et al. 2020. Journal of Virology. 93(20):e00719-19.. PubMed Van der Meer JMR, et al. 2021. Oncoimmunology. 10:1981049. PubMed\u003cbr\u003e\nRRID: AB_2721524 (BioLegend Cat. No. 342611) AB_2721525 (BioLegend Cat. No. 342612)\u003cbr\u003e\nStructure: HLA family, Ig superfamily, 42 kD\u003cbr\u003e\nDistribution: Broadly expressed\u003cbr\u003e\nFunction: Mediate cell recognition by NK cells\u003cbr\u003e\nLigand\/Receptor: CD94\/NKG2\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Sullivan LC, et al. 2008. Tissue Antigen. 72:415. 2. Koller BH, et al. 1988. J. Immunol. 141:897.\u003cbr\u003e\nGene ID: 3133\u003cbr\u003e\nUniProt: View information about HLA-E on UniProt.org\u003cbr\u003e\nClone: 3D12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: HLA class I histocompatibility antigen, alpha chain E, HLAE\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862392164521,"sku":"342611","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-342611","provider":"Iright","version":"1.0","type":"link"}