{"product_id":"biolegend-343111","title":"Biolegend, 343111, Purified anti-human CD7 (Maxpar® Ready) Antibody, 100μg","description":"\u003cp\u003eCD7 is a 40 kD type I transmembrane glycoprotein also known as gp40. It is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL) and some acute myeloid leukemia (AML) cells. CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 costimulation can induce cytokine secretion and modulate cellular adhesion.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: KG1a cell line\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 1.0 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested CyTOF® - Verified\u003cbr\u003e\nRecommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.\u003cbr\u003e\nApplication Notes: Additional reported (for the relevant formats) applications include proteogenomics2.\u003cbr\u003e\nAdditional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV:White Cell Differentiation Antigens. Oxford University Press. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)\u003cbr\u003e\nProduct Citations: Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed NULL, et al. 2022. Cell. 185:916. PubMed\u003cbr\u003e\nRRID: AB_2563761 (BioLegend Cat. No. 343111)\u003cbr\u003e\nStructure: Ig superfamily, type I transmembrane glycoprotein, 40 kD\u003cbr\u003e\nDistribution: T cells, NK cells, thymocytes, hematopoietic progenitors, myeloid leukemic cell subsets\u003cbr\u003e\nFunction: T cell activation\u003cbr\u003e\nLigand\/Receptor: Cytoplasmic domain associates with P13-kinase\u003cbr\u003e\nCell Type: Hematopoietic stem and progenitors, Leukemia, NK cells, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego. 2. Stillwell R, et al. 2001. Immunol. Res. 24:31. 3. Rabinowich H, et al. 1994. J. Immunol. 152:517.\u003cbr\u003e\nGene ID: 924\u003cbr\u003e\nUniProt: View information about CD7 on UniProt.org\u003cbr\u003e\nClone: CD7-6B7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: IV T-164\u003cbr\u003e\nOther Names: gp40\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?\u003cbr\u003e\nA: We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.\u003c\/p\u003e\n\n\u003cp\u003ehttp:\/\/techsupport.fluidigm.com\/\u003cbr\u003e\nQ: Can I use Maxpar® Ready format clones for flow cytometry staining?\u003cbr\u003e\nA: We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.\u003cbr\u003e\nQ: I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.\u003cbr\u003e\nA: We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http:\/\/techsupport.fluidigm.com\/\u003cbr\u003e\nQ: Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?\u003cbr\u003e\nA: The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862989033641,"sku":"343111","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-343111","provider":"Iright","version":"1.0","type":"link"}