{"product_id":"biolegend-343513","title":"Biolegend, 343513, APC\/Cyanine7 anti-human CD34 Antibody, 25tests","description":"\u003cp\u003eCD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem\/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem\/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Cynomolgus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: The 581 antibody recognizes the class III group epitope which is resistant to sialidase\/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press. Felschow DM, et al. 2001. Blood 97:3768. Rudin CE, et al. 1997. Br. J. Haematol. 97:488. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC) Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)\u003cbr\u003e\nProduct Citations: Kfoury Y, et al. 2021. Cancer Cell. 39:1464. PubMed Dickerson KM, et al. 2022. Blood Cancer Discov. 3:240. PubMed Baudet A, et al. 2022. Bio Protoc. 12:e4353. PubMed Takayama N, et al. 2020. Cell Stem Cell. 28(3):488-501.e10. PubMed Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed Shukla S, et al. 2021. Nat Chem Biol. 17:784. PubMed Mori Y, et al. 2015. Proc Natl Acad Sci U S A. 112: 9638-9643. PubMed Nguyen MAT, et al. 2020. Immunohorizons. 0.465277778. PubMed Lal A, et al. 2021. Nat Commun. 1507:12. PubMed Bigley V, et al. 2011. J Exp Med. 208:227. PubMed Montefiori LE, et al. 2021. Cancer Discov. 11:2846. PubMed Zhou X, et al. 2017. J Vasc Surg. 65:1161. PubMed Olesinski EA, et al. 2021. STAR Protocols. 2(2):100461. PubMed Dorrell C, et al. 2016. Nat Commun. 7:11756. PubMed Hofmann M, et al. 2012. Leukemia. 26:1288. PubMed Kohn L, et al. 2014. J Immunol. 192:5050. PubMed Bigley V, et al. 2015. J Leukoc Biol. 97:627. PubMed Isobe T, et al. 2022. Nat Commun. 13:4501. PubMed Lee J, et al. 2015. J Exp Med. 212:385. PubMed Breton G, et al. 2015. J Exp Med. 212:401. PubMed Berent-Maoz B, et al. 2015. PLoS One. 10:125414. PubMed Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed\u003cbr\u003e\nRRID: AB_1877169 (BioLegend Cat. No. 343513) AB_1877168 (BioLegend Cat. No. 343514)\u003cbr\u003e\nStructure: 105-120 kD single chain mucin-like glycoprotein\u003cbr\u003e\nDistribution: Hematopoietic stem\/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts\u003cbr\u003e\nFunction: Cell adhesion\u003cbr\u003e\nLigand\/Receptor: L-selectin, E-selectin\u003cbr\u003e\nCell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Krause DS, et al. 1996. Blood 87:1. 2. Puri KD, et al. 1995. J. Cell Biol. 131:261. 3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley \u0026amp; Sons Inc, Hoboken New Jersey.\u003cbr\u003e\nGene ID: 947\u003cbr\u003e\nUniProt: View information about CD34 on UniProt.org\u003cbr\u003e\nClone: 581\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V MA27\u003cbr\u003e\nOther Names: Gp105-120, My10\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862445805737,"sku":"343513","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-343513","provider":"Iright","version":"1.0","type":"link"}