{"product_id":"biolegend-344004","title":"Biolegend, 344004, PE anti-human CD73 (Ecto-5'-nucleotidase) Antibody, 100tests","description":"\u003cp\u003eCD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include:immunofluorescence3. Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Nakamura T, et al. 1993. J. Immunol. 151:6933. Liao J, et al. 2011. J Endod. 37:1217. PubMed Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF) Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block)\u003cbr\u003e\nProduct Citations: Xin Y, et al. 2021. Stem Cell Res Ther. 49:12. PubMed Zhu H, et al. 2022. Cell Transplant. 31:9636897221106996. PubMed Ishiuchi N, et al. 2023. Stem Cell Res Ther. 14:121. PubMed Sonoda S, et al. 2022. STAR Protoc. 3:101386. PubMed Liu C, et al. 2022. Regen Ther. 21:192. PubMed Costa MHG, et al. 2021. Biotechnol J. 16:e2000389. PubMed Labedz-Maslowska A, et al. 2021. Cells. 10: . PubMed Polisetti N, et al. 2022. Bio Protoc. 12: . PubMed Liu C, et al. 2022. BMC Oral Health. 22:536. PubMed Robb KP, et al. 2022. Front Immunol. 13:972095. PubMed Ryzhov S, et al. 2019. J Am Heart Assoc. 8:e010874. PubMed Shi L, et al. 2021. Stem Cell Res Ther. 12:147. PubMed Huang X, et al. 2019. Oxid Med Cell Longev. 2019:1305049. PubMed Gao G, et al. 2022. Am J Cancer Res. 12:1934. PubMed Sung SE, et al. 2021. Int J Mol Sci. 22:. PubMed Saze Z, et al. 2013. Blood. 122:9. PubMed Ali S, et al. 2018. Purinergic Signal. 14:371. PubMed Sung SE, et al. 2021. Asian Spine J. Online ahead of print.. PubMed Zhang J, et al. 2022. Int J Mol Sci. 23:. PubMed Kicova M, et al. 2020. Neoplasma. 67:933. PubMed Zhang M, et al. 2013. Urology. 81:465. PubMed Jungwirth N et al. 2018. Journal of molecular neuroscience : MN. 65(1):60-73 . PubMed Stocco E, et al. 2020. Front Cell Dev Biol. 0.515972222. PubMed Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed Blázquez-Prunera A, et al. 2017. Stem Cell Res Ther. 10.1186\/s13287-017-0552-z. PubMed Wu L, et al. 2018. Oncol Lett. 15:9507. PubMed Hasmim M, et al. 2022. Front Immunol. 13:982821. PubMed Kolodziej M, et al. 2019. Adipocyte. 0.509722222. PubMed Peng H, et al. 2020. Ann Transl Med. 0.927777778. PubMed Zhang Y, et al. 2019. Aging (Albany NY). 11:12641. PubMed de Sousa BM, et al. 2021. NPJ Regen Med. 6:80. PubMed Yoshida R, et al. 2022. Cancer Res. :. PubMed Okamura G, et al. 2020. Int J Mol Sci. 22:00. PubMed Ross NT, et al. 2020. Nature Chemical Biology. 16(1):50-59.. PubMed Sun T, et al. 2019. Haematologica. 105:661. PubMed Yao X, et al. 2022. Bioeng Transl Med. 7:e10250. PubMed Cantoni C, et al. 2021. Oncoimmunology. 1879530:10. PubMed He H, et al. 2019. J Cell Mol Med. 23:4139. PubMed Wang W, et al. 2022. Int J Mol Sci. 23:. PubMed Labedz-Maslowska A, et al. 2020. Int J Mol Sci. 21:. PubMed Vaka R, et al. 2022. Stem Cell Res Ther. 13:20. PubMed Schultheiß C, et al. 2020. Immunity. 53(2):442-455.e4.. PubMed Lee EC, et al. 2020. Int J Mol Sci. 21:00. PubMed Hong Y, et al. 2020. Aging Cell. 19:e13128. PubMed Dong N, et al. 2022. World J Stem Cells. 14:556. PubMed Bi R, et al. 2020. Osteoarthritis Cartilage. 28:842. PubMed Chijimatsu R, et al. 2022. Regen Ther. 21:52. PubMed\u003cbr\u003e\nRRID: AB_1877224 (BioLegend Cat. No. 344003) AB_2298698 (BioLegend Cat. No. 344004)\u003cbr\u003e\nStructure: GPI-linked 5'-nucleotidase, 70 kD\u003cbr\u003e\nDistribution: Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells\u003cbr\u003e\nFunction: Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology, Stem Cells\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley \u0026amp; Sons Inc, Publication. 2. Airas L and Jalkanen S, et al. 1996. Blood 88:1755. 3. Gutensohn W, et al. 1995. Cell Immunol. 161:213. 4. Airas L, et al. 1995. J. Exp. Med. 182:1603.\u003cbr\u003e\nGene ID: 4907\u003cbr\u003e\nUniProt: View information about CD73 on UniProt.org\u003cbr\u003e\nClone: AD2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V B-CD73.3\u003cbr\u003e\nOther Names: Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862530773161,"sku":"344004","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-344004","provider":"Iright","version":"1.0","type":"link"}