{"product_id":"biolegend-345039","title":"Biolegend, 345039, GoInVivo™ Purified anti-human CD366 (Tim-3) Antibody, 25mg","description":"\u003cp\u003eCD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes\/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.\u003cbr\u003e\n25mg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human Tim-3 fusion protein\u003cbr\u003e\nFormulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.\u003cbr\u003e\nEndotoxin Level: Less than 1.0 EU\/mg of the protein (\u0026lt; 0.1 pg\/µg of the protein) as determined by the LAL test.\u003cbr\u003e\nPreparation: The GoInVivo™ antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg\/mL and 3 mg\/mL.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.\u003cbr\u003e\nApplication: FC - Quality tested Costim, Block - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.\u003cbr\u003e\nApplication Notes: Additional reported applications (for relevant formats of this clone) include: costimulation1 (clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking2,3.\u003cbr\u003e\nAdditional Product Notes: GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hastings WD, et al. 2009. Eur. J. Immunol. 39:2492. (Costim) Jones RB, et al. 2008. J. Exp. Med. 205:2763. (Block) Klibi J, et al 2009. Blood 113:1957. (FC, Block)\u003cbr\u003e\nProduct Citations: Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed Meng Q, et al. 2021. J Immunother Cancer. 9:. PubMed\u003cbr\u003e\nRRID: AB_2566293 (BioLegend Cat. No. 345038) AB_2566294 (BioLegend Cat. No. 345039) AB_2566295 (BioLegend Cat. No. 345040) AB_2566086 (BioLegend Cat. No. 345035) AB_2566087 (BioLegend Cat. No. 345036) AB_2566088 (BioLegend Cat. No. 345037)\u003cbr\u003e\nStructure: Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains\u003cbr\u003e\nDistribution: Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells\u003cbr\u003e\nFunction: Plays a role in regulating macrophage activation, T cell apoptosis and immune tolerance\u003cbr\u003e\nLigand\/Receptor: Galectin-9\u003cbr\u003e\nCell Type: Dendritic cells, Monocytes, T cells, Th1, Tregs\u003cbr\u003e\nBiology Area: Immunology, Inhibitory Molecules\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Hafler DA and Kuchroo V. 2008. J. Exp. Med. 205:2699. 2. Zhu C, et al. 2005. Nat. Immunol. 6:1245. 3. Wang F, et al. 2009. Immunobiology 214:342.\u003cbr\u003e\nGene ID: 84868\u003cbr\u003e\nUniProt: View information about CD366 on UniProt.org\u003cbr\u003e\nClone: F38-2E2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863082487977,"sku":"345039","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-345039","provider":"Iright","version":"1.0","type":"link"}