{"product_id":"biolegend-346003","title":"Biolegend, 346003, PE anti-human CD169 (Sialoadhesin, Siglec-1) Antibody, 25tests","description":"\u003cp\u003eCD169, also known as Siglec-1 and Sialoadhesin (Sn), is a 210 kD type I single membrane-spanning glycoprotein. It is the largest member of the Siglec family, consisting of 1709 amino acids and belonging to the immunoglobulin superfamily. CD169 is expressed by macrophages and dendritic cells. By its affinity to α2,3-linked sialic acid, it is involved in macrophage binding to different cell types such as granulocytes, monocytes, NK, B and T cells. Several CD169 counter receptors, such as CD227 on human breast cancer cells, CD43 on T cells and CD206 on macrophages, have been reported.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human Rhinovirus (HRV14) infected, monocyte derived-DCs\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: Western blotting and inhibition of erythrocyte-rosetting with cells expressing CD169.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kirchberger S, et al. 2005. J. Immunol. 175:1145. Schrauf C, et al. 2009. J. Immunol. 183:4440.\u003cbr\u003e\nProduct Citations: Silvin A, et al. 2020. Cell. 182:1401. PubMed van der Wijst MGP, et al. 2021. Sci Transl Med. 13:eabh2624. PubMed Ostendorf L, et al. 2021. Scientific Reports. 11(1):10299. PubMed OhAinle M et al. 2018. eLife. 7 pii: e39823. PubMed Heikema A, et al. 2013. Infect Immun . 81:2095. PubMed Saito Y, et al. 2015. Cancer Immunol Res. 3: 1356 - 1363. PubMed Perez–Zsolt D, et al. 2019. Front Immunol. 0.989583333. PubMed Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed Okoye AA, et al. 2018. Nat Med. 24:1430. PubMed Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed Kim WK, et al. 2015. AIDS Res Hum Retroviruses . 31: 696-706. PubMed Benet S, et al. 2021. J Extracell Vesicles. 10:e12046. PubMed\u003cbr\u003e\nRRID: AB_2189030 (BioLegend Cat. No. 346003) AB_2189029 (BioLegend Cat. No. 346004)\u003cbr\u003e\nStructure: Type I single membrane-spanning lectin, 1709 amino acids, 210 kD, belongs to the immunoglobulin superfamily\u003cbr\u003e\nDistribution: Macrophages, dendritic cells\u003cbr\u003e\nFunction: Adhesion molecule\u003cbr\u003e\nInteraction: α-2,3-linked sialic acid\u003cbr\u003e\nLigand\/Receptor: CD227, CD43 and CD206\u003cbr\u003e\nBioactivity: Mediates sialic-acid dependent binding to granulocytes, monocytes, NK, B and T cells.\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Macrophages\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules, Siglec Molecules\u003cbr\u003e\nAntigen References: 1. Xiong YS, et al. 2009. Clin. Biochem. 42:1057. 2. Varki A, et al. 2009. Glycoconj J. 26:231. 3. Rempel H, et al. 2008. PLoS One. 3:e1967. 4. Crocker PR, et al. 2001. Trends Immunol. 22:337. 5. Hartnell A, et al. 2001. Blood 97:288.\u003cbr\u003e\nGene ID: 6614\u003cbr\u003e\nUniProt: View information about CD169 on UniProt.org\u003cbr\u003e\nClone: 7-239\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Sialic acid binding Ig-like lectin 1 (Siglec-1)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862482309289,"sku":"346003","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-346003","provider":"Iright","version":"1.0","type":"link"}