{"product_id":"biolegend-347316","title":"Biolegend, 347316, Alexa Fluor® 647 anti-human CD115 (CSF-1R) Antibody, 100tests","description":"\u003cp\u003eCSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitinylation of intracellular residues. The natural ligands of CD115 include M-CSF and IL-34. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes\/macrophages, plasmacytoid and conventional dendritic cells, and osteoclasts.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: C-fms transduced Kirsten strain murine sarcoma virus transformed NRK cells.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1151.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed\u003cbr\u003e\nRRID: AB_2687249 (BioLegend Cat. No. 347315) AB_2687250 (BioLegend Cat. No. 347316)\u003cbr\u003e\nStructure: Single-pass type I membrane protein and is 150 kD.\u003cbr\u003e\nDistribution: Monocytes\/macrophages, plasmacytoid and conventional dendritic cells, and osteoclasts.\u003cbr\u003e\nLigand\/Receptor: Macrophage Colony Stimulating Factor (M-CSF) and IL-34.\u003cbr\u003e\nCell Type: Dendritic cells, Macrophages, Monocytes, Osteoclasts\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Sherr CJ, et al. 1989. Blood 73:1786 2. Roussel MF, et al. 1991. Nature 353:361. 3. Roussel MF, et al. 1989 P. Natl. Acad. Sci. USA 86:7924.\u003cbr\u003e\nGene ID: 1436\u003cbr\u003e\nUniProt: View information about CD115 on UniProt.org\u003cbr\u003e\nClone: 9-4D2-1E4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V MA199\u003cbr\u003e\nOther Names: M-CSFR,CSF-1R, FMS, CSF-1 Receptor\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: Why do I have a weak CD115 staining?\u003cbr\u003e\nA: It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation.  Pre-treatment with EDTA and low temperatures (2 - 8°C) helps in maintaining surface expression of CD115. In addition, brief fixation of the cells with Fixation Buffer (Cat. No. 420801) for 10 minutes will block CD115 internalization.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862496858281,"sku":"347316","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-347316","provider":"Iright","version":"1.0","type":"link"}