{"product_id":"biolegend-350010","title":"Biolegend, 350010, PerCP\/Cyanine5.5 anti-human CD134 (OX40) Antibody, 100tests","description":"\u003cp\u003eCD134, also known as OX40 and TNFRSF4, is a 50 kD type I transmembrane glycoprotein. It is a member of the TNF receptor family. OX40 is expressed on activated T lymphocytes including Th1, Th2, Th17, and Treg cells. The interaction of OX40 with OX40L results in B cell proliferation and antibody secretion, regulation of primary T cell expansion, and T cell survival. OX40 influences the size of the T cell memory pool and regulation of CD4 + T cell tolerance.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Chimpanzee\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: HTLV 1-transformed HUT 102 cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, immunohistochemical staining2,3of paraffin embedded7 and frozen tissue sections, ELISA4, and functional assay5. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Latza U, et al. 1994. Eur. J. Immunol. 24:677. (WB, IP) Durkop H, et al. 1995. Brit. J. Haematol. 91:927. (IHC) Durkop H, et al. 1997. Brit. J. Haematol. 98:863. (IHC) Willett B, et al. 2007. J. Virol. 81:9665. (ELISA) Li M and Zhang Y. et al. 2005. Cell. Mol. Immunol. 2:467. (FA) Gloviczki ML, et al. 2012. Clin. J. Am. Soc. Nephrol. 8:546. PubMed Domingos PL, et al. 2012. An. Bras. Dermatol. 87:851. (IHC)\u003cbr\u003e\nProduct Citations: Schroeder JC, et al. 2020. Cancers (Basel). :12. PubMed Vorkas CK, et al. 2022. J Immunol. 208:1042. PubMed Koutsakos M, et al. 2022. Immunity. 55:1316. PubMed Juno JA, et al. 2020. Nat Med. 26:1428. PubMed Kalimuddin S, et al. 2021. Med. 2(6):682-688.e4. PubMed Ohue Y, et al. 2014. Clin Cancer Res. 20:5052. PubMed\u003cbr\u003e\nRRID: AB_10720986 (BioLegend Cat. No. 350009) AB_10719224 (BioLegend Cat. No. 350010)\u003cbr\u003e\nStructure: 50 kD, TNF receptor family\u003cbr\u003e\nDistribution: Activated T cells\u003cbr\u003e\nFunction: Receptor for OX40 ligand, co-stimulatory signal for T cell proliferation and survival.\u003cbr\u003e\nLigand\/Receptor: OX40L (CD252)\u003cbr\u003e\nCell Type: T cells, Tregs\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Smith CA, et al. 1994. Cell. 76:959. 2. Chen AL, et al. 1999. Immunity. 11:689. 3. Croft M. 2010. Annu. Rev. Immunol. 28:57. 4. Withers DR, et al. 2009. J. Immunol. 183:5079. 5. Klinger M, et al. 2009. J. Immunol. 182:4581.\u003cbr\u003e\nGene ID: 7293\u003cbr\u003e\nUniProt: View information about CD134 on UniProt.org\u003cbr\u003e\nClone: Ber-ACT35 (ACT35)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: ACT35 antigen, tumor necrosis factor receptor superfamily member 4 (TNFRSF4)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862905933993,"sku":"350010","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-350010","provider":"Iright","version":"1.0","type":"link"}