{"product_id":"biolegend-350101","title":"Biolegend, 350101, Purified anti-human CD294 (CRTH2) Antibody, 25μg","description":"\u003cp\u003eCD294, also known as CRTH2, is a seven-transmembrane protein coupled with heterotrimeric G proteins. CRTH2 is the prostaglandin D2 receptor and is expressed by Th2 cells, eosinophils, and basophils. CD294 prevents the apoptosis of Th2 cells and mediates the chemotaxis of CRTH2 expressing cells to the sites of allergic inflammation, such as the asthmatic lung.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Rat cell line TART\/B19-12.10 transfected with human CRTH2\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This product is sold under license agreement with BML, Inc for research use only.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Nagata K, et al. 1999. J. Immunol. 162:1278. (FC) De Fanis U, et al. 2007. Blood 109:4343. (FC) Tsuda H, et al. 2001. Clin. Exp. Immunol. 123:105. (FC) Hamada K, et al. 2004. Allergology International 53:179. (FC)\u003cbr\u003e\nProduct Citations: Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed Stras SF, et al. 2020. Developmental Cell. 51(3):357-373.e5.. PubMed van Montfoort N, et al. 2018. Cell. 175:1744. PubMed Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed Kim ML, et al. 2018. Circulation. 138:2648. PubMed Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed\u003cbr\u003e\nRRID: AB_10639863 (BioLegend Cat. No. 350102)\u003cbr\u003e\nStructure: Seven-transmembrane protein coupled with G proteins\u003cbr\u003e\nDistribution: Effector T helper 2 (Th2) cells, eosinophils, basophils\u003cbr\u003e\nFunction: Chemotaxis of eosinophils, basophils and Th2 cells, prevention of apoptosis in Th2 cells\u003cbr\u003e\nInteraction: Heterotrimeric G proteins\u003cbr\u003e\nLigand\/Receptor: Prostaglandin D2\u003cbr\u003e\nCell Type: Basophils, Eosinophils, Th2\u003cbr\u003e\nBiology Area: Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Luster AD and Tager AM. 2004. Nat. Rev. Immunol. 4:711. 2. Monneret G, et al. 2001. Blood 98:1942. 3. Xue L, et al. 2009. J. Immunol. 182:7580. 4. Schratl P, et al. 2007. J. Immunol. 179:4792.\u003cbr\u003e\nGene ID: 11251\u003cbr\u003e\nUniProt: View information about CD294 on UniProt.org\u003cbr\u003e\nClone: BM16\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Chemoattractant receptor-homologous molecule expressed on Th2 cells, GPR44, G-protein coupled receptor 44, DP2\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862911996073,"sku":"350101","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-350101","provider":"Iright","version":"1.0","type":"link"}