{"product_id":"biolegend-353216","title":"Biolegend, 353216, FITC anti-human CD197 (CCR7) Antibody, 100tests","description":"\u003cp\u003eCCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: CCR7-transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nAdditional Product Notes: View more applications data for this product in our Scientific Poster Library.\u003cbr\u003e\nProduct Citations: Adams S, et al. 2012. Clin Cancer Res. 18:6748. PubMed Vyasamneni R, et al. 2023. Cell Rep Methods. 3:100388. PubMed Si W, et al. 2023. iScience. 26:106529. PubMed Kim JH, et al. 2020. Sci Rep. 10:1835. PubMed Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed Conforti A, et al. 2021. PLoS One. 16:e0259894. PubMed Hearnden R, et al. 2021. STAR Protocols. 2(2):100422. PubMed Rubio-Pérez C, et al. 2021. Nat Commun. 1503:12. PubMed Dan H, et al. 2020. Mol Oncol. 14:795. PubMed Zebley CC, et al. 2021. Cell Rep. 37:109796. PubMed Vanshylla K, et al. 2022. Cell Host Microbe. 30:69. PubMed Tarke A, et al. 2021. Cell Reports Medicine. 2(7):100355. PubMed Kim D, et al. 2020. Nat Med. 26:236. PubMed Peluso MJ, et al. 2021. Cell Rep. 36:109518. PubMed Einkauf KB, et al. 2019. J Clin Invest. 129:988. PubMed Rebouças A, et al. 2021. Microorganisms. 9:. PubMed Zebley CC, et al. 2021. Cell Rep. 37:110079. PubMed Mudd PA, et al. 2022. Cell. 185:603. PubMed Yu ED, et al. 2022. Cell Host Microbe. 30:1269. PubMed Jiang C, et al. 2020. Nature. 261:585. PubMed Einkauf KB, et al. 2022. Cell. 185:266. PubMed Lazarski CA, et al. 2020. Cytotherapy. . PubMed Fu Y, et al. 2021. Cell Reports. 36(2):109344. PubMed Qin H, et al. 2015. Blood. 126: 629 - 639. PubMed Sehgal R, et al. 2022. Front Immunol. 13:828949. PubMed Schouest B, et al. 2021. J Virol. 95:. PubMed Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed Wang B, et al. 2018. Mol Ther Nucleic Acids. 0.548611111. PubMed Sasawatari S, et al. 2020. J Immunol. 1373:204. PubMed Yu ED, et al. 2022. Cell Host Microbe. . PubMed Hu W, et al. 2022. Mater Today Bio. 14:100224. PubMed\u003cbr\u003e\nRRID: AB_10945291 (BioLegend Cat. No. 353215) AB_10916386 (BioLegend Cat. No. 353216)\u003cbr\u003e\nStructure: Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.\u003cbr\u003e\nDistribution: T cells, B cells, NK, dendritic cells.\u003cbr\u003e\nFunction: The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.\u003cbr\u003e\nLigand\/Receptor: CCL19 and CCL21.\u003cbr\u003e\nCell Type: B cells, Dendritic cells, NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Yanagihara S, et al. 1998. J. Immunol. 161:3096. 2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610. 3. Reif K, et al. 2002. Nature 416:94. 4. Nakata B, et al. 2008. Oncology 74:69. 5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed 6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed 7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed\u003cbr\u003e\nGene ID: 1236\u003cbr\u003e\nUniProt: View information about CD197 on UniProt.org\u003cbr\u003e\nClone: G043H7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BLR2, CDw197, EBI1, CMKBR7\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866096914601,"sku":"353216","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-353216","provider":"Iright","version":"1.0","type":"link"}