{"product_id":"biolegend-353448","title":"Biolegend, 353448, PE\/Fire™ 640 anti-human CD196 (CCR6) Antibody, 25tests","description":"\u003cp\u003eCCR6, also known as CD196, is a chemokine receptor that is expressed on immature dendritic cells, B lymphocytes, and memory T cells. CCR6 binds CCL20, although members of the β defensin family also bind CCR6 with a lower affinity. CCR6 positive cells, and its ligand CCL20, have been detected in numerous organs, especially the secondary lymphoid organ. CCL20 is selectively made by the follicle-associated epithelium (FAE) overlying Peyers Patches (PPs) and isolated lymphoid follicles (ILFs). CCL20 contributes to the recruitment of CCR6-expressing B cells to these structures. In humans, CCR6 can function to mediate arrest of T cells on dermal endothelial cells and is highly expressed on T cells resident in both normal and psoriatic skin. CCR6 and\/or CCL20 have been implicated in the pathogenesis of rheumatoid arthritis and inflammatory bowel disease. Human T cells that are able to produce IL-17 express CCR6. It suggests that CCL20 and CCR6 have a role in inflammatory diseases by recruiting Th17 cells to target tissues.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: CCR6-transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Fire™ 640 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PE\/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nRRID: AB_2924552 (BioLegend Cat. No. 353448) AB_2924552 (BioLegend Cat. No. 353449)\u003cbr\u003e\nStructure: Chemokine receptor, GPCR, seven transmembrane receptor\u003cbr\u003e\nDistribution: Immature dendritic cells, B lymphocytes, memory T cells, Th17 cells\u003cbr\u003e\nFunction: Host defense, important for recruitment of B cells to secondary lymphoid organs, mediates arrest of T cells on dermal endothelial cells\u003cbr\u003e\nLigand\/Receptor: CCL20 (MIP-3α)\u003cbr\u003e\nCell Type: B cells, Dendritic cells, T cells, Th17\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Zaballos A, et al. 1996. Biochem. Bioph. Res. Co. 227:846. 2. Yang D, et al. 1999. Science 286:525. 3. MacDonald KG, et al. 2007. Am. J. Pathol. 170:1229. 4. Homey B, et al. 2000. J. Immunol. 164:6621. 5. Hirota K, et al. 2007. J. Exp. Med. 204:2803. 6. Singh SP, et al. 2008. J. Immunol. 180:214.\u003cbr\u003e\nGene ID: 1235\u003cbr\u003e\nUniProt: View information about CD196 on UniProt.org\u003cbr\u003e\nClone: G034E3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BN-1, CKR6, DCR2, CKRL3, DRY-6, GPR29, CKR-L3, CMKBR6, GPRCY4, STRL22, GPR-CY4\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866095440041,"sku":"353448","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-353448","provider":"Iright","version":"1.0","type":"link"}