{"product_id":"biolegend-353801","title":"Biolegend, 353801, Purified anti-human CD370 (CLEC9A\/DNGR1) Antibody, 25μg","description":"\u003cp\u003eCD370 (CLEC9A), also known as DNGR1, is a type II transmembrane protein consisting of a single extracellular C-type lectin domain (CTLD) that is connected to the transmembrane domain by a stalk region, and an intracellular cytoplasmic tail with immunoreceptor tyrosine-based activation motif (ITAM). In humans, CLEC9A is strictly expressed on BDCA3-positive conventional dendritic cells (cDC) and CD16 - monocytes. CLEC9A recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed\u003cbr\u003e\nRRID: AB_10983070 (BioLegend Cat. No. 353802)\u003cbr\u003e\nStructure: Type II transmembrane glycoprotein; consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM like motif, a 21 aa transmembrane segment, a 185 aa extracellular domain (ECD) that contains a stalk region, and one C-type lectin domain (CTLD).\u003cbr\u003e\nDistribution: Dendritic subsets including plasmacytoid dendritic cells.\u003cbr\u003e\nFunction: Cross-present antigen to CTL, phagocytosis necrotic cells.\u003cbr\u003e\nInteraction: Necrotic cells.\u003cbr\u003e\nLigand\/Receptor: Ubiquitous preformed acid-labile protein associated ligand(s).\u003cbr\u003e\nCell Type: Dendritic cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, MHC Antigens\u003cbr\u003e\nAntigen References: 1. Poulin LF, et al. 2010. J. Exp. Med. 207:1261. 2. Sancho D, et al. 2009. Nature 458:899. 3. Huysamen C, et al. 2008. J. Biol. Chem. 283:16693.\u003cbr\u003e\nGene ID: 283420\u003cbr\u003e\nUniProt: View information about CD370 on UniProt.org\u003cbr\u003e\nClone: 8F9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: C-type lectin family member 9A, CD370\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862496137385,"sku":"353801","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-353801","provider":"Iright","version":"1.0","type":"link"}