{"product_id":"biolegend-354702","title":"Biolegend, 354702, Purified anti-human CD301 (CLEC10A) Antibody, 100μg","description":"\u003cp\u003eCD301, also known as CLEC10A, macrophage galactose\/N-acetylgalactosamine (GalNAc) specific lectin (MGL), DCASGPR, and HML, is a 40 kD type II transmembrane glycoprotein, which belongs to the C-type lectin family. Human CD301 consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment, and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region.  CD301 is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages. The expression level is upregulated by immunosuppressant dexamethasone. Human CD301 has an exclusive specificity for rare terminal GalNAc structures, which are revealed on the tumor-associated mucin MUC1 and CD45 (RA, RB, and RC but not RO isoforms). This interaction inhibits effector T cell activation and induces their apoptosis. CD301 also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant CD301 (extracellular domain)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: H037G3 antibody binding is divalent cation dependent.\u003cbr\u003e\nProduct Citations: Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed\u003cbr\u003e\nRRID: AB_11219398 (BioLegend Cat. No. 354701) AB_11218998 (BioLegend Cat. No. 354702)\u003cbr\u003e\nStructure: 40 kD type II transmembrane glycoprotein, consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region\u003cbr\u003e\nDistribution: Immature myeloid dendritic cells, activated macrophages\u003cbr\u003e\nFunction: Cell adhesion, cell-cell signaling, glycoprotein turnover, inflammation, immune response\u003cbr\u003e\nInteraction: Carbohydrate determinants, GP envelope glycoprotein on Marburg and Ebola viruses\u003cbr\u003e\nLigand\/Receptor: Glycans containing terminal GalNAc moieties\u003cbr\u003e\nCell Type: Dendritic cells, Macrophages\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Van Vliet SJ, et al. 2006. Nat. Immunol. 7:1200. 2. Higashi N, et al. 2002. J. Biol. Chem. 277:20686. 3. Tsuiji M, et al. 2002. J. Biol. Chem. 277:28892. 4. Valladeau J, et al. 2001. J. Immunol. 167:5767. 5. Suzuki N, et al. 1996. J. Immunol. 156:128.\u003cbr\u003e\nGene ID: 10462\u003cbr\u003e\nUniProt: View information about CD301 on UniProt.org\u003cbr\u003e\nClone: H037G3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Macrophage galactose\/Nacetylgalactosamine (GalNAc) specific lectin, MGL, DCASGPR, HML CLECSF13, CLECSF14, HML2\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866100912297,"sku":"354702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-354702","provider":"Iright","version":"1.0","type":"link"}