{"product_id":"biolegend-356903","title":"Biolegend, 356903, PE anti-human CD185 (CXCR5) Antibody, 25tests","description":"\u003cp\u003eCD185, also known as CXCR5, is a 42 kD G-protein coupled receptor with seven transmembrane regions. CXCR5 is expressed by mature B cells, follicular helper T cells, Burkitt’s lymphoma cells and a subset of neurons, and mediates cell migration to the B cell follicles in the secondary lymphoid organs. The ligand of CXCR5 is CXCL13 (BLC).\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human CXCR5-transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nProduct Citations: Carre C, et al. 2021. iScience. 24:102970. PubMed He T, et al. 2023. Immun Inflamm Dis. 11:e759. PubMed Lagou V et al. 2018. Cell reports. 25(3):798-810 . PubMed Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed Serra–Peinado C, et al. 2019. Nat Commun. 10:3705. PubMed Kaneko N, et al. 2020. Cell. 183(1):143-157.e13. PubMed Vanshylla K, et al. 2022. Cell Host Microbe. 30:69. PubMed Seo IH, et al. 2021. Immune Netw. 21:e17. PubMed Woodruff M, et al. 2020. medRxiv. . PubMed Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed Schultheiß C, et al. 2020. Immunity. 53(2):442-455.e4.. PubMed Platteel ACM, et al. 2018. Front Immunol. 1.544444444. PubMed Li G, et al. 2021. Mol Ther Oncolytics. 22:507. PubMed\u003cbr\u003e\nRRID: AB_2561812 (BioLegend Cat. No. 356903) AB_2561813 (BioLegend Cat. No. 356904)\u003cbr\u003e\nStructure: G-protein coupled receptor, 7 transmembrane regions, 42 kD\u003cbr\u003e\nDistribution: Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.\u003cbr\u003e\nFunction: Mediates cell migration to the B cell follicles in the secondary lymphoid organs\u003cbr\u003e\nLigand\/Receptor: CXCL13\u003cbr\u003e\nCell Type: B cells, Tfh\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Ma CS, et al. 2012. J. Exp. Med. 209:1241. 2. León B, et al. 2012. Nat. Immunol. 13:681. 3. Crotty S. 2011. Annu. Rev. Immunol. 29:621. 4. Kerfoot SM, et al. 2011. Immunity 34:947. 5. Sáez de Guinoa J, et al. 2011. Blood 118:1560.\u003cbr\u003e\nGene ID: 643\u003cbr\u003e\nUniProt: View information about CD185 on UniProt.org\u003cbr\u003e\nClone: J252D4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BLR1, MDR15\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866112315561,"sku":"356903","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-356903","provider":"Iright","version":"1.0","type":"link"}