{"product_id":"biolegend-362108","title":"Biolegend, 362108, PE\/Cyanine7 anti-human CCX-CKR (CCRL1) Antibody, 100tests","description":"\u003cp\u003eHuman CCX-CKR, also known as CCRL1, is a 40 kD, G-protein-coupled, seven transmembrane receptor. CCRL1 is a decoy receptor for CCL2, CCL8, CCL13, CCL19, CCL21 and CCL25 that regulates chemotaxis of lymphocytes and cancer cells. Chemokine binding by CCX-CKR causes its internalization, reducing levels of available chemokine, and thus regulating migration of lymphocytes and cancerous cells. CCX-CKR is expressed by stromal cells of skin-draining lymph nodes, thymic epithelial cells, and immature dendritic cells (DCs). A low CCX-CKR expression in tumor cells correlates with a poor survival rate of breast cancer patients.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Full length CCX-CKR cDNA\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications for the relevant formats include: immunoprecipitation of CCX-CKR from cell lysates1, induction of CCX-CKR internalization1 and immunofluorescence1. 13E11 recognizes an epitope located within the first 14 amino acids of CCX-CKR.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Takatsuka, S. et al. 2011. J. Pharmacol. Toxicol. Methods. 63:250. (IF, IP, Agonist)\u003cbr\u003e\nRRID: AB_2728346 (BioLegend Cat. No. 362107) AB_2728347 (BioLegend Cat. No. 362108)\u003cbr\u003e\nStructure: G-protein-coupled seven transmembrane receptor, 40 kD.\u003cbr\u003e\nDistribution: Stromal cells of skin-draining lymph nodes, thymic epithelial cells, immature DCs.\u003cbr\u003e\nFunction: C-C type chemokine scavenger and decoy receptor, reduces chemokine availability by sequestering them inside the cell.\u003cbr\u003e\nInteraction: β-arrestin.\u003cbr\u003e\nLigand\/Receptor: CCL2, CCL8, CCL13, CCL19, CCL21, and CCL25.\u003cbr\u003e\nCell Type: Dendritic cells, Epithelial cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Watts AO, et al. 2013. J. Biol. Chem. 288:7169. 2. Comerford I, et al. 2010. Blood. 116:4130.\u003cbr\u003e\nGene ID: 51554\u003cbr\u003e\nUniProt: View information about CCX-CKR on UniProt.org\u003cbr\u003e\nClone: 13E11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: ACKR4, CC-CKR-11, VSHK1, PPR1, CCBP2, CCR11, CKR-11, CCXCKR\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860801507497,"sku":"362108","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-362108","provider":"Iright","version":"1.0","type":"link"}