{"product_id":"biolegend-362603","title":"Biolegend, 362603, PE anti-His Tag Antibody, 100tests","description":"\u003cp\u003eThis antibody was raised against a linear 8XHis tag. It reacts with His-tagged protein with C, N or internal 6XHis tags or longer. It can be used for western blotting to detect linear His repeats (6XHis or more). It can also be used for ICFC and ICC staining of His-tagged proteins.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat, All species\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: KLH-conjugated His-tag peptide.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: This antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC. Clone J095G46 has been verified for immunocytochemistry (ICC).\u003cbr\u003e\nProduct Citations: Tasker C, et al. 2021. AAPS J. 23:26. PubMed Li Z, et al. 2019. Cell Mol Immunol. 0.897222222. PubMed Gall CLM, et al. 2023. Bio Protoc. 13:e4613. PubMed Lubin JH, et al. 2023. JCI Insight. . PubMed Golubovskaya V, et al. 2023. Cancers (Basel). 15:. PubMed Liu L, et al. 2022. Sci Transl Med. 14:eabn6859. PubMed Huang J, et al. 2021. Immunity. 54(4):673-686.e4. PubMed Wei C, et al. 2020. Nat Metab. 1.049305556. PubMed Le Gall CM, et al. 2021. Bioconjug Chem. 32:301. PubMed Yang J, et al. 2020. Nature. 586:572. PubMed Zhu QY, et al. 2021. Nat Commun. 12:6624. PubMed Tong P, et al. 2021. Cell. :. PubMed Huang HC, et al. 2021. EBioMedicine. 74:103712. PubMed Scheu AHA, et al. 2021. Nat Commun. 0.997916667. PubMed Pivniouk V, et al. 2022. J Allergy Clin Immunol. 149:923. PubMed Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed Long Y, et al. 2021. Biochem Biophys Res Commun. 571:152. PubMed van der Schoot JMS, et al. 2019. Sci Adv. 5:eaaw1822. PubMed Sokal A, et al. 2021. Immunity. S1074-7613:00396. PubMed Alberts A, et al. 2020. Front Immunol. 11:596103. PubMed Wang R, et al. 2021. Immunity. 54:1611. PubMed Zhang M, et al. 2022. iScience. 25:104490. PubMed\u003cbr\u003e\nRRID: AB_2563634 (BioLegend Cat. No. 362603)\u003cbr\u003e\nStructure: 6 repeat His amino acid\u003cbr\u003e\nFunction: Epitope tag\u003cbr\u003e\nBiology Area: Cell Biology, Immunology\u003cbr\u003e\nAntigen References: Zhao C, et al. 2010. Anal Biochem. 399:237 Salichs E, et al. 2009. PLoS Genet. 5:e1000397 Terpe K. 2003. Appl. Microbiol. Biotechnol. 60:523 Lindner, P, et al. 1997. Biotechniques. 22:140 Burritt JB, et al. 1995. J. Biol. Chem. 270:16974. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about His Tag on UniProt.org\u003cbr\u003e\nClone: J095G46\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Hexa histidine-tag, 6xHis-tag, 6XHis, 6His\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866109661353,"sku":"362603","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-362603","provider":"Iright","version":"1.0","type":"link"}