{"product_id":"biolegend-362701","title":"Biolegend, 362701, Purified anti-human\/mouse CD3ε (activation epitope) Antibody, 50μg","description":"\u003cp\u003eThe CD3ε chain of the CD3-TCR complex is a type I membrane protein that undergoes conformational change following ligation of the TCR-CD3 complex.  This conformational change leads to exposure of the proline rich sequence in CD3ε and results in recruitment of Nck. It is expressed by all mature T cells and forms a heteromeric complex with CD3 α\/β or γ\/δ and ζ chains. It is important in T cell development, and deficiency of this gene has been associated with severe immunodeficiency.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Purified denatured CD3 proteins from human thymus\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ICFC - Quality tested WB, IHC-P - Verified in vivo cell tracking - Reported in the literature, not verified in house ICC, IHC, IP - Reported by the developer, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For Western blotting, the suggested use of this reagent is 1.0 - 4.0 µg per mL. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone APA1\/1 recognizes an activation-dependent intracellular epitope of the CD3ε chain. Additional reported applications (for the relevant formats of this clone) include: immunofluorescent staining of paraformaldehyde fixed sections1, in vivo tracking of T cells 2, and Western blotting3. The LEAF purified format is recommended for in vivo studies.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Salmerón A, et al. 1991. J. Immunol. 147:3047. (ICFC, IF) Risueño RM, et al. 2005. Blood. 106:601. (IF, in vivo tracking, Mouse Reactivity) Risueño RM, et al. 2008. PLos One. 3:e1747. (WB) Gil D, et al. 2005. J. Exp. Med. 201:517. Risueño RM, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:9625.\u003cbr\u003e\nProduct Citations: Srivastava R, et al. 2023. Antib Ther. 253:6. PubMed Kuai Y, et al. 2020. Theranostics. 5.099305556. PubMed\u003cbr\u003e\nRRID: AB_2563713 (BioLegend Cat. No. 362701) AB_2563714 (BioLegend Cat. No. 362702)\u003cbr\u003e\nStructure: Type I membrane protein, part of the TCR-CD3 complex. 207 amino acids, predicted molecular weight of 23 kD.\u003cbr\u003e\nDistribution: Mature T cells.\u003cbr\u003e\nFunction: Important role in T cell development and in linking antigen recognition with intracellular signaling pathways.\u003cbr\u003e\nInteraction: Interacts with Nck.\u003cbr\u003e\nLigand\/Receptor: MHC antigen.\u003cbr\u003e\nCell Type: T cells\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nMolecular Family: CD Molecules, TCRs\u003cbr\u003e\nAntigen References: 1. Savage PD, et al. 1988. Cytogenet. Cell. Genet. 49:289. 2. DeJarnette JB, et al. 1998. Proc. Natl. Acad. Sci USA. 95:14909. 3. Wang B, et al. 1999. J. Immunol. 162:88. 4. Szymczak AL, et al. 2005. J. Immunol. 175:270. 5. Borroto A, et al. 2014. J. Immunol. 192:2042.\u003cbr\u003e\nGene ID: 916\u003cbr\u003e\nUniProt: View information about CD3epsilon on UniProt.org\u003cbr\u003e\nClone: APA1\/1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD3e molecule, epsilon (CD3-TCR complex), CD3e antigen, CD3 epsilon\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866451005609,"sku":"362701","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-362701","provider":"Iright","version":"1.0","type":"link"}