{"product_id":"biolegend-366201","title":"Biolegend, 366201, Purified anti-PTEN Antibody, 25μg","description":"\u003cp\u003ePTEN was discovered as a tumor suppressor with lipid and protein phosphatase activity. Heterozygous loss of PTEN in mice contribute to the development of a variety of types of cancer, suggesting that insufficient PTEN activity results in increased cancer susceptibility. PTEN negatively regulates the PI3K\/AKT pathway by dephosphorylating the second messenger phosphoinositide 3,4,5-triphosphate (PIP3) produced by phosphoinositide 3-kinase (PI3K), which results in inhibition of abnormal cell proliferation. Despite the hydrolysis function of PIP3, the nuclear population of PTEN possesses greater tumor suppressive activities, regulates gene expression, differentiation, and cell cycle progression.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human PTEN protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone failed western blot, IHC, and ICC testing during product development. We therefore do not recommend this product for any of these applications.\u003cbr\u003e\nRRID: AB_2922580 (BioLegend Cat. No. 366201)\u003cbr\u003e\nStructure: PTEN is a 403 amino acid protein with a predicted molecular weight of 47 kD.\u003cbr\u003e\nDistribution: Ubiquitous\/ Nucleus (monoubiquitinated form), Cytoplasm (nonubiquitinated form)\u003cbr\u003e\nFunction: Hydrolase, Protein Phosphatase\u003cbr\u003e\nInteraction: Interacts with MAIG2, MAIG3, MAST1, MAST3, NEDD4, NDFIP1, NDFIP2, USP7, ROCK1, and XIAP. The unphosphorylated form interacts with AIP1, DLG1 and MAS.T2.\u003cbr\u003e\nLigand\/Receptor: Lipid-binding\u003cbr\u003e\nBiology Area: Angiogenesis, Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Cell Cycle\/DNA Replication, Signal Transduction, Transcription Factors\u003cbr\u003e\nMolecular Family: Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: Carracedo A, et al. 2011. Cancer Res. 71:629-33. Jiang BH and Liu LZ. 2009. Adv Cancer Res. 102:19-65. Mao JH, et al. 2008. Science 321:1499-502. Weng LP, et al. 2001. Hum. Mol. Genet. 10:599-604. Wrighton KH. 2011. Nat Rev Cancer. 11:154. Wang X, et al. 2007. Cell 128:129-39.\u003cbr\u003e\nGene ID: 5728\u003cbr\u003e\nUniProt: View information about PTEN on UniProt.org\u003cbr\u003e\nClone: W20041H\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BZS, TEP1, PTEN1, PTENbeta, Phosphatase and tensin homolog, MMAC1, CWS1, DEC, GLM2, MHAM\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866418401449,"sku":"366201","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-366201","provider":"Iright","version":"1.0","type":"link"}