{"product_id":"biolegend-368811","title":"Biolegend, 368811, PE\/Cyanine7 anti-human TdT Antibody, 25tests","description":"\u003cp\u003eTerminal deoxynucleotidyl transferase (TdT), also known as DNA nucleotidylexotransferase (DNTT) or terminal transferase, is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. TdT is a specialized DNA polymerase expressed early in pre-B and pre-T lymphoid cell development, and in acute lymphoblastic leukemia\/lymphoma cells. TdT contributes to the generation of junctional diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant full length human TdT\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.\u003cbr\u003e\nRRID: AB_3097578 (BioLegend Cat. No. 368811) AB_3097578 (BioLegend Cat. No. 368812)\u003cbr\u003e\nDistribution: pre-B and pre-T lymphocytes, malignant pre-B and pre-T lymphocytes.\u003cbr\u003e\nFunction: Catalyzes the addition of deoxynucleotides to non-germ line elements at the junctions of rearranged Ig heavy chain and T cell receptor gene segments and thus produces antigen receptor diversity.\u003cbr\u003e\nInteraction: PCNA, LPTS-RP1, LPTS-RP2, histones H2A, H2B, H3, and H4.\u003cbr\u003e\nLigand\/Receptor: This process doesn't need a complementary DNA template.\u003cbr\u003e\nCell Type: B cells, T cells\u003cbr\u003e\nBiology Area: Cell Biology, DNA Repair\/Replication, Immunology\u003cbr\u003e\nMolecular Family: Enzymes and Regulators\u003cbr\u003e\nAntigen References: 1. Yunk L, et al. 2009. J. Immunol. 183:452. 2. Kedzierska K, et al. 2008. J. Immunol. 181:2556. 3. Fuller SA, et al. 1985. Biochem. J. 231:105. 4. Feeney AJ, et al. 2001. J. Immunol. 167:3486. 5. Komori, T, et al. 1993. Science 261:1171.\u003cbr\u003e\nGene ID: 1791\u003cbr\u003e\nUniProt: View information about TdT on UniProt.org\u003cbr\u003e\nClone: 41A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Terminal-Deoxynucleotidyl Transferase, DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal transferase, DNTT\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862401601705,"sku":"368811","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-368811","provider":"Iright","version":"1.0","type":"link"}