{"product_id":"biolegend-369501","title":"Biolegend, 369501, Purified anti-ERK1\/2 Phospho (Thr202\/Tyr204) Antibody, 25μg","description":"\u003cp\u003eERK1\/2 are members of mitogen-activated kinases (MAPKs) of serine\/threonine protein kinases. ERK1\/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1\/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:500-1: 5000 diluted (0.1-1.0 µg\/ml). For flow cytometric staining, the suggested use of this reagent is ≤ 5.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone 6B8B69 was found to strongly cross-react with mouse ERK1\/2 Phospho (Thr202\/Tyr204) when tested in-house on C57BL\/6 mouse splenocytes.\u003cbr\u003e\nProduct Citations: Recabal A, et al. 2020. Journal of Neurochemistry. 156(2):182-199. PubMed\u003cbr\u003e\nRRID: AB_2721734 (BioLegend Cat. No. 369501) AB_2721735 (BioLegend Cat. No. 369502)\u003cbr\u003e\nStructure: ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.\u003cbr\u003e\nDistribution: Cytoplasm and the nucleus.\u003cbr\u003e\nFunction: ERK1\/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1 and ERK2 are phosphorylated at Thr202 and Tyr204; and in turn transduce signals to downstream substrates.\u003cbr\u003e\nInteraction: ERK1\/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.\u003cbr\u003e\nCell Type: Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology\u003cbr\u003e\nMolecular Family: Postsynaptic proteins, Phospho-Proteins, Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: 1. Futran AS, et al. 2013. Curr. Biol. 23:R972. 2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320. 3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299. 4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320. 5. Torii S, et al. 2004. J. Biochem. 136:557. 6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.\u003cbr\u003e\nGene ID: 5594\u003cbr\u003e\nUniProt: View information about ERK1\/2 on UniProt.org\u003cbr\u003e\nClone: 6B8B69\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: P44\/42 MAP (motogen activated protein) Kinase, Extracelluar signal-regulated kinase (ERK)\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864375906473,"sku":"369501","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-369501","provider":"Iright","version":"1.0","type":"link"}