{"product_id":"biolegend-369604","title":"Biolegend, 369604, PE anti-human CD152 (CTLA-4) Antibody, 100μg","description":"\u003cp\u003eCD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Regulatory T cells express high levels of CTLA-4. CTLA-4 (CD152) is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CTLA-4 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.  Mutations in the CTLA-4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases. A transcript of the CTLA-4 gene that may represent a native soluble form of CTLA-4 (sCTLA-4) showed that eleven of twenty patients with autoimmune thyroid disease (ATD) had a high concentration of sCTLA-4, whereas only 1 of 30 apparently healthy volunteers contained measurable levels. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA-4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Extracellular domain of human CTLA-4 and constant regions of the human IgG heavy chain (CTLA-4\/IgG)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested FC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Based on in-house testing, we do not recommend using clone BNI3 for immunohistochemistry of paraffin-embedded tissue section.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Linsley PS, et al. 1992. J. Exp. Med. 176:1595. Bonzheim I, et al. 2008. Am. J. Clin. Pathol. 130:613.\u003cbr\u003e\nProduct Citations: Preglej T, et al. 2023. Front Immunol. 14:1096096. PubMed Zhang Y, et al. 2019. Cell Res. 29:609. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed Makdasi E, et al. 2020. Mol Cancer Ther. 1.147916667. PubMed Harper JL, et al. 2020. Nat Med. 519:26. PubMed Luo Y, et al. 2021. Nat Commun. 12:3913. PubMed Karlsson J, et al. 2020. Nat Commun. 1.773611111. PubMed Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed\u003cbr\u003e\nRRID: AB_2566796 (BioLegend Cat. No. 369603) AB_2566797 (BioLegend Cat. No. 369604)\u003cbr\u003e\nStructure: Ig superfamily and 33 kD.\u003cbr\u003e\nDistribution: Activated T cells and B cells.\u003cbr\u003e\nFunction: Negative regulator of T cell activation.\u003cbr\u003e\nInteraction: Antigen presenting cells, such as dendritic cells.\u003cbr\u003e\nLigand\/Receptor: B7-1 (CD80), B7-2 (CD86).\u003cbr\u003e\nCell Type: B cells, T cells\u003cbr\u003e\nBiology Area: Immunology, Inhibitory Molecules\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Kuiper HM, et al. 1995. J. Immunol. 155:1776. 2. Castan J, et al. 1997. Immunology 90:265. 3. Lee CC, et al. 2009. Pediatr. Allergy Immunol. 20:624. 4. Pistillo MP, et al. 2003. Blood 101:202. 5. Tan PH, et al. 2005. Blood. 106:2936. 6. Steiner K, et al. 2001. Clin. Exp. Immunol. 126:143.\u003cbr\u003e\nGene ID: 1493\u003cbr\u003e\nUniProt: View information about CD152 on UniProt.org\u003cbr\u003e\nClone: BNI3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CTLA4, Cytotoxic T-Lymphocyte Antigen 4, CTLA-4\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862915469481,"sku":"369604","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-369604","provider":"Iright","version":"1.0","type":"link"}