{"product_id":"biolegend-369607","title":"Biolegend, 369607, PerCP\/Cyanine5.5 anti-human CD152 (CTLA-4) Antibody, 25tests","description":"\u003cp\u003eCD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Regulatory T cells express high levels of CTLA-4. CTLA-4 (CD152) is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CTLA-4 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.  Mutations in the CTLA-4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases. A transcript of the CTLA-4 gene that may represent a native soluble form of CTLA-4 (sCTLA-4) showed that eleven of twenty patients with autoimmune thyroid disease (ATD) had a high concentration of sCTLA-4, whereas only 1 of 30 apparently healthy volunteers contained measurable levels. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA-4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Extracellular domain of human CTLA-4 and constant regions of the human IgG heavy chain (CTLA-4\/IgG)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality testedFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Based on in-house testing, we do not recommend using clone BNI3 for immunohistochemistry of paraffin-embedded tissue section.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Linsley PS, et al. 1992. J. Exp. Med. 176:1595. Bonzheim I, et al. 2008. Am. J. Clin. Pathol. 130:613.\u003cbr\u003e\nProduct Citations: Cao B, et al. 2022. Nat Commun. 13:6203. PubMed Kim DH, et al. 2021. eLife. 10:00. PubMed Acharya A, et al. 2020. J Virol. 95:e01657. PubMed van Montfoort N, et al. 2018. Cell. 175:1744. PubMed Reustle A, et al. 2022. Clin Transl Med. 12:e883. PubMed Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed\u003cbr\u003e\nRRID: AB_2629673 (BioLegend Cat. No. 369607) AB_2629674 (BioLegend Cat. No. 369608)\u003cbr\u003e\nStructure: Ig superfamily and 33 kD.\u003cbr\u003e\nDistribution: Activated T cells and B cells.\u003cbr\u003e\nFunction: Negative regulator of T cell activation.\u003cbr\u003e\nInteraction: Antigen presenting cells, such as dendritic cells.\u003cbr\u003e\nLigand\/Receptor: B7-1 (CD80), B7-2 (CD86).\u003cbr\u003e\nCell Type: B cells, T cells\u003cbr\u003e\nBiology Area: Immunology, Inhibitory Molecules\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Kuiper HM, et al. 1995. J. Immunol. 155:1776. 2. Castan J, et al. 1997. Immunology 90:265. 3. Lee CC, et al. 2009. Pediatr. Allergy Immunol. 20:624. 4. Pistillo MP, et al. 2003. Blood 101:202. 5. Tan PH, et al. 2005. Blood. 106:2936. 6. Steiner K, et al. 2001. Clin. Exp. Immunol. 126:143.\u003cbr\u003e\nGene ID: 1493\u003cbr\u003e\nUniProt: View information about CD152 on UniProt.org\u003cbr\u003e\nClone: BNI3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CTLA4, Cytotoxic T-Lymphocyte Antigen 4, CTLA-4\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862916026537,"sku":"369607","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-369607","provider":"Iright","version":"1.0","type":"link"}