{"product_id":"biolegend-369612","title":"Biolegend, 369612, APC anti-human CD152 (CTLA-4) Antibody, 100tests","description":"\u003cp\u003eCD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Regulatory T cells express high levels of CTLA-4. CTLA-4 (CD152) is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CTLA-4 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.  Mutations in the CTLA-4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases. A transcript of the CTLA-4 gene that may represent a native soluble form of CTLA-4 (sCTLA-4) showed that eleven of twenty patients with autoimmune thyroid disease (ATD) had a high concentration of sCTLA-4, whereas only 1 of 30 apparently healthy volunteers contained measurable levels. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA-4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Extracellular domain of human CTLA-4 and constant regions of the human IgG heavy chain (CTLA-4\/IgG)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality testedFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For intracellular flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Based on in-house testing, we do not recommend using clone BNI3 for immunohistochemistry of paraffin-embedded tissue section.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Linsley PS, et al. 1992. J. Exp. Med. 176:1595. Bonzheim I, et al. 2008. Am. J. Clin. Pathol. 130:613.\u003cbr\u003e\nProduct Citations: Yuan H, et al. 2022. J Clin Exp Hematop. 62:52. PubMed Gan X, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200879119. PubMed Lu T, et al. 2023. Cancer Immunol Immunother. :. PubMed Lee PS, et al. 2022. MAbs. 14:2024642. PubMed Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed Navarrete-Bernal MGC, et al. 2020. Front Oncol. 1.254166667. PubMed Wang X, et al. 2020. Exp Ther Med. 19:3698. PubMed Franchini DM et al. 2019. Cell reports. 26(1):94-107 . PubMed Gamradt S, et al. 2021. iScience. 24:103312. PubMed\u003cbr\u003e\nRRID: AB_2632873 (BioLegend Cat. No. 369611) AB_2632874 (BioLegend Cat. No. 369612)\u003cbr\u003e\nStructure: Ig superfamily and 33 kD.\u003cbr\u003e\nDistribution: Activated T cells and B cells.\u003cbr\u003e\nFunction: Negative regulator of T cell activation.\u003cbr\u003e\nInteraction: Antigen presenting cells, such as dendritic cells.\u003cbr\u003e\nLigand\/Receptor: B7-1 (CD80), B7-2 (CD86).\u003cbr\u003e\nCell Type: B cells, T cells\u003cbr\u003e\nBiology Area: Immunology, Inhibitory Molecules\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Kuiper HM, et al. 1995. J. Immunol. 155:1776. 2. Castan J, et al. 1997. Immunology 90:265. 3. Lee CC, et al. 2009. Pediatr. Allergy Immunol. 20:624. 4. Pistillo MP, et al. 2003. Blood 101:202. 5. Tan PH, et al. 2005. Blood. 106:2936. 6. Steiner K, et al. 2001. Clin. Exp. Immunol. 126:143.\u003cbr\u003e\nGene ID: 1493\u003cbr\u003e\nUniProt: View information about CD152 on UniProt.org\u003cbr\u003e\nClone: BNI3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CTLA4, Cytotoxic T-Lymphocyte Antigen 4, CTLA-4\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862915076265,"sku":"369612","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-369612","provider":"Iright","version":"1.0","type":"link"}