{"product_id":"biolegend-371010","title":"Biolegend, 371010, Brilliant Violet 421™ anti-human Aiolos Antibody, 100tests","description":"\u003cp\u003eAiolos, also known as Ikaros family zinc finger 3 (IKZF3), is a 58kD zinc finger protein involved in hematopoetic-specific transcriptional activation. Aiolos is involved in regulation of gene expression in B lymphocytes through chromatin remodeling and formation of homodimers and heterodimers with other members of the Ikaros family. Deletion of Aiolos has been reported in pre-B-acute lymphocytic leukemia (B-ALL).\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial Aiolos recombinant protein (1-130aa) expressed in E. Coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended. This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).\u003cbr\u003e\nRRID: AB_2616874 (BioLegend Cat. No. 371009) AB_2616875 (BioLegend Cat. No. 371010)\u003cbr\u003e\nStructure: 58kD, zinc finger protein.\u003cbr\u003e\nDistribution: T and B lymphocytes.\u003cbr\u003e\nFunction: Hematopoetic-specific transcriptional activator and chromatin remodeler.\u003cbr\u003e\nInteraction: Forms heterodimers with Ikaros family members IKZF4 and IKZF5.\u003cbr\u003e\nCell Type: B cells, T cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Chromatin Remodeling\/Epigenetics, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Zhuang Y, et al. 2015. Oncol. Rep. 33:1319. 2. Ma S, et al. 2008. Blood. 111:1396. 3. Billot K, et al. 2010. Leuk. Res. 34:289. 4. Morgan B, et al. 2004. EMBO J. 16:2004. 5. Wang JH, et al. 1998. Immunity. 9:543-53. 6. Hosokawa Y, et al. 1999. Genomics. 61:326.\u003cbr\u003e\nGene ID: 22806\u003cbr\u003e\nUniProt: View information about Aiolos on UniProt.org\u003cbr\u003e\nClone: 14C4C97\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IKZF3, AIO, ZNFN\/A3\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864349495465,"sku":"371010","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-371010","provider":"Iright","version":"1.0","type":"link"}