{"product_id":"biolegend-371207","title":"Biolegend, 371207, Brilliant Violet 421™ anti-human CD357 (GITR) Antibody, 25tests","description":"\u003cp\u003eGITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptor superfamily member (also known as AITR and TNFRSF18). GITR is expressed on activated lymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain of GITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation and TCR-mediated apoptosis, and to break immunological self-tolerance. GITR binds GITRL and is involved in the development of regulatory T cells and to regulate the activity of Th1 subsets.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human GITR-Fc chimera\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nProduct Citations: Schroeder JC, et al. 2020. Cancers (Basel). :12. PubMed Franchini DM et al. 2019. Cell reports. 26(1):94-107 . PubMed Suzuki S, et al. 2020. Cancer Sci. 1943:111. PubMed\u003cbr\u003e\nRRID: AB_2616850 (BioLegend Cat. No. 371207) AB_2616851 (BioLegend Cat. No. 371208)\u003cbr\u003e\nStructure: TNFR superfamily, 234-amino acid type I transmembrane protein, 25 kD.\u003cbr\u003e\nDistribution: Activated lymphocytes.\u003cbr\u003e\nFunction: Interacts with TRAF's 1-3, but not TRAF 5 or 6, mediates NF-κB activation through the TRAF2\/NIK pathway.\u003cbr\u003e\nLigand\/Receptor: GITRL\u003cbr\u003e\nCell Type: Lymphocytes, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. van der Werf N, et al. 2011. J. Immunol. 187:1411. 2. Shimizu J, et al. 2002. Nat. Immunol. 3:135. 3. McHugh RS, et al. 2002. Immunity 16:311. 4. Kwon B, et al. 1999. J. Biol. Chem. 274:6056.\u003cbr\u003e\nGene ID: 8784\u003cbr\u003e\nUniProt: View information about CD357 on UniProt.org\u003cbr\u003e\nClone: 108-17\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: TNFRSF18, Activation-inducible TNFR, AITR, Glucocorticoid-inducible TNFR\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860777193641,"sku":"371207","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-371207","provider":"Iright","version":"1.0","type":"link"}