{"product_id":"biolegend-372722","title":"Biolegend, 372722, Biotin anti-human TIGIT (VSTM3) Antibody, 100μg","description":"\u003cp\u003eT cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM, is a 26 kD, type I transmembrane protein and is a member of the PVR (poliovirus receptor) family of immunoglobulin-like domain containing proteins. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. TIGIT is a negative regulator of NK and T cell activation. Expression of TIGIT is associated with decreased functionality of CD8 T cells in chronic viral infection and tumors. TIGIT also promotes the differentiation of tolerogenic phenotype in dendritic cells with an increased secretion of IL-10 and a diminished production of IL-12.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant Human TIGIT.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing.This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).Additional reported applications (for the relevant formats) include: Blocking1.\u003cbr\u003e\nAdditional Product Notes: View more applications data for this product in our Scientific Poster Library.Watch a Scientific Poster video of this product.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Stamm H, et al. 2018. Oncogene. Pubmed\u003cbr\u003e\nRRID: AB_2687252 (BioLegend Cat. No. 372722)\u003cbr\u003e\nStructure: 26kD; type I transmembrane protein, Ig-like V-type domain, ITIM motif.\u003cbr\u003e\nDistribution: Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells.\u003cbr\u003e\nFunction: Cell signaling, negative regulation of T cells, T cell tolerance, T cell anergy.\u003cbr\u003e\nLigand\/Receptor: CD155 (PVR), CD112 (PVRL2, NECTIN-2).\u003cbr\u003e\nCell Type: NK cells, T cells, Tfh, Tregs\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Inhibitory Molecules, Signal Transduction\u003cbr\u003e\nMolecular Family: Adhesion Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. 106:17858. 2. Yu X, et al. 2009. Nat. Immunol. 10:48. 3. Johnston R, et al. 2014. Cancer Cell. 26:923.\u003cbr\u003e\nGene ID: 201633\u003cbr\u003e\nUniProt: View information about TIGIT on UniProt.org\u003cbr\u003e\nClone: A15153G\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864390389929,"sku":"372722","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-372722","provider":"Iright","version":"1.0","type":"link"}