{"product_id":"biolegend-373207","title":"Biolegend, 373207, APC anti-human HLA-F Antibody, 25tests","description":"\u003cp\u003eA 42kD member of non-classical or class Ib human MHC family. It exhibits low allelic polymorphism and shorter cytoplasmic tail due to absence of exon seven. It is expressed irrespective of bound peptide. It is primarily found intracellularly on Endoplasmic Reticulum and Golgi membrane and its surface expression increases in correlation with classical MHC-I up regulation in activated monocytes, B, T, and NK cells. It is involved in inflammatory responses. It stabilizes the unstable MHC-I Open Conformers (OC) and facilitates uptake of extracellular antigen for cross presentation.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Complex mixed with IFA, complex without adjuvant.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ishitani A, et al. 2003. J. Immunol. 171:1376.\u003cbr\u003e\nProduct Citations: Peng W, et al. 2023. Cells. 12: . PubMed Nguyen MAT, et al. 2020. Immunohorizons. 0.465277778. PubMed\u003cbr\u003e\nRRID: AB_2783287 (BioLegend Cat. No. 373207) AB_2783288 (BioLegend Cat. No. 373208)\u003cbr\u003e\nStructure: 42 kD Single pass type I membrane protein, heterodimer of an alpha and a Beta chain.\u003cbr\u003e\nDistribution: B, NK, monocyte and T cells.\u003cbr\u003e\nFunction: MHC-I OC stabilization, antigen cross presentation.\u003cbr\u003e\nLigand\/Receptor: KIR3DS1, KIR3DS4, KIR3DL2, ILT2, ILT4, TAP.\u003cbr\u003e\nCell Type: B cells, Monocytes, NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: Goodridge JP, et al. 2013. J. Immunol. 191:1567. Lepin EJ, et al. 2000. Eur. J. Immunol. 30:3552. Burian A,et al. 2016. PLoS One 11:e0163297. Lee N, et al. 2010. Eur. J. Immunol. 40:2308.\u003cbr\u003e\nGene ID: 3134\u003cbr\u003e\nUniProt: View information about HLA-F on UniProt.org\u003cbr\u003e\nClone: 3D11\/HLA-F\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: HLAF, CDA12, HLA-5.4, HLA-CDA12\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862391148713,"sku":"373207","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-373207","provider":"Iright","version":"1.0","type":"link"}