{"product_id":"biolegend-377002","title":"Biolegend, 377002, Purified anti-human CD276 (B7-H3) Antibody, 100μg","description":"\u003cp\u003eB7-H3, assigned as CD276, is a type I transmembrane protein and shares 20-27% amino acid identity with other B7 family members. Human B7-H3 has a single extracellular variable-type immunoglobulin (IgV)-IgC domain, a signature intracellular domain, and an additional isoform, known as 4Ig-B7-H3, containing nearly exact tandem duplication of the IgV-IgC domain and most likely caused by exon duplication. B7-H3 mRNA is broadly expressed in normal tissues whereas its protein expression is relatively rare. The expression of B7-H3 is induced on T cells, natural killer (NK) cells, and antigen-presenting cells (APCs), including dendritic cells (DCs) and macrophages. It can be upregulated during the maturation from monocytes to DCs, or during the interaction between DCs and regulatory T cells. B7-H3 has been shown to be a co-stimulatory molecule that inhibits T-cell responses. B7-H3 has also been identified to bind TLT-2 involved in the intracellular signaling pathway.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human retinoblastoma cell line, WERI-RB-1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Tryfonidou M, et al. 2014. Cytometry A. 85:379-81. (FC) Autenrieth S, et al. 2015. Clin Transl Immunology. 4:e50. (FC) Buhring H J, et al. 2007. Ann N Y Acad Sci. 1106:262-71. (FC)\u003cbr\u003e\nRRID: AB_2910432 (BioLegend Cat. No. 377002)\u003cbr\u003e\nStructure: Type I transmembrane protein with extracellular variable-type immunoglobulin domain and intracellular domain\u003cbr\u003e\nDistribution: Professional APCs, including B cells, dendritic cells, macrophages, and mesenchymal stem cells\u003cbr\u003e\nLigand\/Receptor: Trem like transcript 2 protein (TLT-2)\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells, Dendritic cells, Macrophages\u003cbr\u003e\nBiology Area: Immuno-Oncology, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Sun M, et al. 2002. J. Immunology. 168:6294-7. Xu J, et al. 2006. Cell Mol Immunol. 3:235-40. Sugawara E, et al. 2020. Autophagy. 16:946-955.\u003cbr\u003e\nGene ID: 80381\u003cbr\u003e\nUniProt: View information about CD276 on UniProt.org\u003cbr\u003e\nClone: W5C4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: 4Ig-B7-H3, B7RP2\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864085909673,"sku":"377002","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-377002","provider":"Iright","version":"1.0","type":"link"}