{"product_id":"biolegend-377117","title":"Biolegend, 377117, PE anti-human CD50 (ICAM-3) Antibody, 25tests","description":"\u003cp\u003eCD50, also known as intercellular adhesion molecule 3 (ICAM-3), is a 120-140 kD type I membrane protein and member of the Ig superfamily. It is broadly expressed on leukocytes, endothelial cells, and Langerhans cells. CD50 provides costimulatory signals for T cells and regulates cell adhesion through interaction with LFA-1 (CD11a\/CD18, α L \/β 2 ) integrin.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Baboon, Cynomolgus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant Human ICAM3\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): deFougerolles AR and Springer TA, et al. 1992. J. Exp. Med. 175:185. (IP, Block) Geijtenbeek T, et al. 2000. Cell. 100:575. (Block) Giannoni E, et al. 2003. J Biol. Chem. 278:36763.\u003cbr\u003e\nRRID: AB_3720617 (BioLegend Cat. No. 377117) AB_3720617 (BioLegend Cat. No. 377118)\u003cbr\u003e\nStructure: Ig superfamily, type I membrane protein, 130 kD\u003cbr\u003e\nFunction: Adhesion, costimulation\u003cbr\u003e\nInteraction: Thymocytes, T and B cells, monocytes, granulocytes, endothelial cells\u003cbr\u003e\nLigand\/Receptor: CD11a\/CD18 (LFA-1), integrin αL\/β2\u003cbr\u003e\nCell Type: B cells, Endothelial cells, Granulocytes, Monocytes, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: Valent P, et al. 2001. Immunol. Rev. 179:74.\u003cbr\u003e\nGene ID: 3385\u003cbr\u003e\nUniProt: View information about CD50 on UniProt.org\u003cbr\u003e\nClone: A22041E\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: ICAM-3\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866076958889,"sku":"377117","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-377117","provider":"Iright","version":"1.0","type":"link"}