{"product_id":"biolegend-377610","title":"Biolegend, 377610, APC anti-human CD28 Antibody, 100tests","description":"\u003cp\u003eCD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human CD28 transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Clone S20013F antibody can completely block the binding of clone CD28.2 on target. Clone S20013F can partially block Clone S20013B on target. Clone S20013F can increase anti-CD3 induced human peripheral T-cell proliferation.\u003cbr\u003e\nRRID: AB_2927985 (BioLegend Cat. No. 377609) AB_2927985 (BioLegend Cat. No. 377610)\u003cbr\u003e\nStructure: Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD\u003cbr\u003e\nDistribution: Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells\u003cbr\u003e\nFunction: T cell costimulation\u003cbr\u003e\nLigand\/Receptor: CD80, CD86\u003cbr\u003e\nCell Type: Lymphocytes, T cells, Tfh, Th1, Th17, Th2, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Adaptive Immunity, Cell Proliferation and Viability, Costimulatory Molecules, Immuno-Oncology, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York. June CH, et al. 1994. Immunol Today. 15:321-31. Linsley PS and Ledbetter JA. 1993. Annu Rev Immunol. 11:191-212.\u003cbr\u003e\nGene ID: 940\u003cbr\u003e\nUniProt: View information about CD28 on UniProt.org\u003cbr\u003e\nClone: S20013F\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T44, Tp44\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866266357929,"sku":"377610","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-377610","provider":"Iright","version":"1.0","type":"link"}