{"product_id":"biolegend-381810","title":"Biolegend, 381810, Brilliant Violet 421™ anti-human HLA-G Recombinant Antibody, 100tests","description":"\u003cp\u003eHLA-G is a nonclassical MHC class I (MHC-Ib) molecule structurally related to MHC class Ia (HLA-A, B, C). There are seven isoforms of this molecule, including membrane bound HLA-G1, -G2, -G3 and -G4 and soluble HLA-G5, -G6, and -G7. HLA-G is primarily expressed on trophoblast cells. Its expression is also found on thymic epithelial cells, cytokine-activated moncytes, macrophages\/dendritic cells during viral infection, and in various tumors. HLA-G exerts its inhibitory functions to regulate immune tolerance via interaction with inhibitory receptors ILT2 (CD85j), ILT4 (CD85d), and KIR2DL4 (CD158d), which is expressed on NK cells, T cells, monocytes, dendritic cells, and B cells. HLA-G is also able to bind CD8α\/α, which may mediate positive and\/or negative selection in the thymus.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Recombinant\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Clone QA19A11 partially blocks the binding of reported clone MEMG\/9.\u003cbr\u003e\nRRID: AB_3717111 (BioLegend Cat. No. 381809) AB_3717111 (BioLegend Cat. No. 381810)\u003cbr\u003e\nStructure: Ig\u003cbr\u003e\nDistribution: Trophoblast cells, thymic epithelial cells, cytokine-activated moncytes, macrophages\/dendritic cells during viral infection, various tumors\u003cbr\u003e\nFunction: Inhibit NK and T cell-mediated cytolysis and T cell proliferation, induce apoptosis of activated CD8+ T cells, induce Th2 cytokine production\u003cbr\u003e\nInteraction: Dendritic cells, Epithelial cells, Macrophages, Monocytes\u003cbr\u003e\nLigand\/Receptor: ILT2 (CD85j), ILT4 (CD85d), KIR2DL4 (CD158d), CD8α\/α\u003cbr\u003e\nCell Type: Dendritic cells, Epithelial cells, Macrophages, Monocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: Hunt JS, et al. 2005. FASEB J. 19:681. Carosella ED, et al. 2008. Blood. 111:4862.\u003cbr\u003e\nGene ID: 3135\u003cbr\u003e\nUniProt: View information about HLA-G on UniProt.org\u003cbr\u003e\nClone: QA19A11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866185945257,"sku":"381810","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-381810","provider":"Iright","version":"1.0","type":"link"}