{"product_id":"biolegend-383207","title":"Biolegend, 383207, PE anti-human CD277 (BTN3A) Recombinant Antibody, 25tests","description":"\u003cp\u003eButyrophilin subfamily 3A (BTN3A), also known as CD277, is a 56 kD type 1 transmembrane glycoprotein. The butyrophilin (BT)\/B7 3A family includes three members, BTN3A1, BTN3A2, and BTN3A3, which share a highly conserved extracellular IgV-like domain. BTN3A is broadly expressed in various immune cells including T cells, B cells, NK cells, monocytes, dendritic cells, endothelial cells, and some tumor cells. It plays a role in T cell activation, regulating their proliferation and release of IFNƳ and other cytokines.  Clone 34-7.Rec has been shown to bind to a V region of BTN3A3 that is shared with BTN3A1 and BTN3A2.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Recombinant\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: pRC-CMV-BTN3A3\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: This product was formerly named “anti-human CD277 (BTN3A1).” The 34-7.Rec clone has been confirmed to recognize BTN3A3. Cross-reactivity with other BTN3A subfamily members has not been tested.\u003cbr\u003e\nRRID: AB_3097513 (BioLegend Cat. No. 383207) AB_3097513 (BioLegend Cat. No. 383208)\u003cbr\u003e\nStructure: 56 kD single pass type I membrane glycoprotein, member of the immunoglobin superfamily\u003cbr\u003e\nDistribution: Cell membrane\u003cbr\u003e\nFunction: Signaling receptor binding, Homodimerization\u003cbr\u003e\nInteraction: TFF1, PEPL, BT3A2, G5E9A7\u003cbr\u003e\nBiology Area: Immuno-Oncology, Inhibitory Molecules\u003cbr\u003e\nMolecular Family: CD Molecules, Tumor Suppressors\u003cbr\u003e\nAntigen References: Vantourout P, et al. 2018. Proc Natl Acad Sci USA. 115:1039-44. Payne KK, et al. 2020. Science. 369: 942-9. Rigau M, et lat. 2020. Science. 367:eaay5516. Yang Y, et al. 2019. Immunity. 50:1043-53.\u003cbr\u003e\nGene ID: 1111910384\u003cbr\u003e\nUniProt: View information about CD277 on UniProt.org\u003cbr\u003e\nClone: 34-7.Rec\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BTF5, BT3.1, BTN3.1, BT3.2, BTF4, BTN3.2, BTF3, BTN3.3\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862375813289,"sku":"383207","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-383207","provider":"Iright","version":"1.0","type":"link"}