{"product_id":"biolegend-383708","title":"Biolegend, 383708, PE anti-human HB-EGF Antibody, 100tests","description":"\u003cp\u003eHeparin-binding EGF-like growth factor (HB-EGF) is a member of the EGF family of growth factors. Human HB-EGF was initially identified as a protein of 22 kD secreted by macrophage-like U937 cells. It belongs to the EGF family of proteins that includes EGF, TGF-α, HB-EGF, epigen, epiregulin, betacellulin, neuroregulin, and tomoregulin. All the EGF family members are synthesized as type I membrane protein precursors, which can undergo proteolytic cleavage at the plasma membrane to release a mature soluble ectodomain. It has been suggested that various metalloproteinases participate in the shedding of HB-EGF, such as MMP-3, MMP-7, ADAM9, ADAM10, ADAM12, and ADAM17. The ectodomain shedding is stimulated by phorbol esters, calcium ionophore, lysophosphatidic acid, and IL-1β. In addition, nardilysin, a metalloendopeptidase of the M16 family, binds HB-EGF and enhances its shedding through activation of TACE (ADAM17). The membrane-anchored HB-EGF acts in cell-cell and cell-extracellular matrix interactions. In addition, the membrane HB-EGF is the receptor for diphtheria toxin that can be internalized and induce apoptotic death. HB-EGF plays a crucial role in cardiac valvulogenesis. Newborn knockout mice have malformed semilunar and atrioventricular heart valves and poorly differentiated lungs. HB-EGF is overexpressed in several types of cancer such as pancreatic carcinoma, ovarian cancer, and gastric carcinoma. HB-EGF is also involved in cancer metastasis and invasion of ovarian cancer, head and neck cancer, and thyroid carcinoma cells.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human HB-EGF, extracellular domain (recombinant)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hamaoka M, et al. 2010. J Biochem. 148:55.\u003cbr\u003e\nRRID: AB_3106178 (BioLegend Cat. No. 383707) AB_3106178 (BioLegend Cat. No. 383708)\u003cbr\u003e\nDistribution: Monocytes, macrophages, T cells, neutrophils, eosinophils, myeloid leukemia blasts, myeloma cells, vascular smooth muscle cells, endothelial cells, and normal or neoplastic epithelial cells\u003cbr\u003e\nFunction: HB-EGF is associated with proliferation and differentiation of stromal cells, apoptosis, and morphogenesis. It participates in inflammation, wound healing, atherosclerotic plaque progression, and cardiac valvulogenesis. TNF-α and IL-1β induces HB-EGF in endothelial cells.\u003cbr\u003e\nInteraction: Fibroblast, smooth muscle cells, monocytes\/macrophages, endothelial cells, astrocytes, keratinocytes, normal and neoplastic epithelial cells\u003cbr\u003e\nLigand\/Receptor: EGFR (ErbB1) and ErbB4; also binds to heparan-sulphate proteoglycans\u003cbr\u003e\nCell Type: Epithelial cells, Macrophages, T cells\u003cbr\u003e\nBiology Area: Angiogenesis, Cancer Biomarkers\u003cbr\u003e\nMolecular Family: Growth Factors\u003cbr\u003e\nAntigen References: Higashiyama S, et al. 1991. Science. 251:936. Hirata M, et al. 2001. Biochem. Biophys. Res. Commun. 283:915. Jackson LF, et al. 2003. EMBO J. 22:2704. Nishi E, et al. 2006. J. Biol. Chem. 281:31164. Yotsumoto F, et al. 2008. Biochem. Biophys. Res. Commun. 365:555. Ota I, et al. 2013. Oncol Rep. 30:1593.\u003cbr\u003e\nGene ID: 1839\u003cbr\u003e\nUniProt: View information about HB-EGF on UniProt.org\u003cbr\u003e\nClone: 3H4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Heparin-binding EGF-like growth factor, HB-EGF-like growth factor\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862400028841,"sku":"383708","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-383708","provider":"Iright","version":"1.0","type":"link"}