{"product_id":"biolegend-384703","title":"Biolegend, 384703, PE anti-human CD125 (IL-5Rα) Antibody, 25tests","description":"\u003cp\u003eCD125, also known as IL5 receptor α-chain, is a 60 kD type I transmembrane protein that binds IL-5 and associates in a heterodimer with the signal-transducing β subunit CD131 to form the IL-5 receptor. The affinity of CD125 for IL-5 is increased when is associated with CD131. The cytoplasmic region of CD125 activates the JAK2\/STAT5 pathway and the kinase Btk. CD125 is expressed by eosinophils, basophils, mast cells, B1 cells and a subset of resting B2 cells and have a central role in the differentiation, activation and survival of these cells. Due to the restricted expression of this molecule, it is considered a potential therapeutic target for allergic inflammatory conditions.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Mouse IL-5Rα transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nRRID: AB_2936755 (BioLegend Cat. No. 384703) AB_2936755 (BioLegend Cat. No. 384704)\u003cbr\u003e\nStructure: Type I transmembrane protein, 60 kD\u003cbr\u003e\nDistribution: Eosinophils, basophils, mast cells, B1 cells, subset of resting B2 cells\u003cbr\u003e\nFunction: Role in eosinophils and basophils differentiation, activation and survival\u003cbr\u003e\nInteraction: JAK2\/STAT, Btk, SOX4\u003cbr\u003e\nLigand\/Receptor: IL-5\u003cbr\u003e\nCell Type: B cells, Basophils, Eosinophils\u003cbr\u003e\nBiology Area: Cell Biology, Cell Proliferation and Viability, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: Buchheit KM, et al. 2020. J Allergy Clin Immunol. 145:1574-1584. Lin X, et al. 2020. Inflammation. 43:1797-1805. Gorski SA, et al. 2019. PLoS One. 14:e0221113. Varricchi G, et al. 2016. Curr Opin Allergy Clin Immunol.16:186-200. Beekman JM. et al. 2009. Blood. 114:3917-27. Lei JT. et al. 2008. J Leukoc Biol. 84:499-509.\u003cbr\u003e\nGene ID: 3568\u003cbr\u003e\nUniProt: View information about CD125 on UniProt.org\u003cbr\u003e\nClone: S20015E\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IL5RA, IL5R, CDw125, HSIL5R3\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866266980521,"sku":"384703","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-384703","provider":"Iright","version":"1.0","type":"link"}