{"product_id":"biolegend-385605","title":"Biolegend, 385605, APC anti-human CD25 Antibody, 25tests","description":"\u003cp\u003eCD25 is a 55 kD transmembrane protein also known as interleukin-2 receptor alpha chain. CD25 in combination with CD122 and CD132 form a high affinity IL-2 receptor that binds to IL-2, playing a crucial role in activation of JAK1\/JAK3 and STAT5 signaling. It is expressed on activated T-Cells as well as on CD4+ T regulatory cells, some progenitor B-cells and activated monocytes.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human CD25 transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Clone S20019C can completely block binding of clone BC96 and clone TÜ69 on target cells and can be blocked by BC96 and by TÜ69.Clone S20019C does not block the binding of clone S20019D and clone M-A251 on target cells.Clone S20019C can block IL-2 binding to receptor.Clone S20019C shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.\u003cbr\u003e\nRRID: AB_3068168 (BioLegend Cat. No. 385605) AB_3068168 (BioLegend Cat. No. 385606)\u003cbr\u003e\nStructure: 55 kD transmembrane protein\u003cbr\u003e\nDistribution: Activated T-Cells, T regulatory Cells, monocytes\u003cbr\u003e\nFunction: IL-2 receptor\u003cbr\u003e\nInteraction: CD122, CD132\u003cbr\u003e\nLigand\/Receptor: IL-2\u003cbr\u003e\nCell Type: Lymphocytes, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Waldmann T. 1991. J Biol. Chem. 266:2681-4. Sakaguchi S, et al. 1995. The Journal of Immunology. 155:1151-64.\u003cbr\u003e\nGene ID: 3559\u003cbr\u003e\nUniProt: View information about CD25 on UniProt.org\u003cbr\u003e\nClone: S20019C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: interleukin-2 receptor alpha chain, interluekin-2 receptor, IL2RA, IL-2Rα\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866267898025,"sku":"385605","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-385605","provider":"Iright","version":"1.0","type":"link"}