{"product_id":"biolegend-388814","title":"Biolegend, 388814, PE\/Dazzle™ 594 anti-human CD8a Antibody, 100tests","description":"\u003cp\u003eCD8α, also known as T8 or Leu2, is a 32-34 kD type 1 transmembrane glycoprotein part of the immunoglobulin superfamily. CD8α is expressed on cytotoxic T cells, NK Cells, as well as a majority off thymocytes. CD8α can form a homodimer (CD8α\/α) or heterodimer (CD8α\/β) with CD8β. CD8α acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8α are important for function: the extracellular Ig superfamily domain binds the α3 domain of MHC Class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human CD8α-transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Dazzle™ 594 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PE\/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone S21010F can completely block the binding of clone HIT8a, clone RPA-T8, and clone SK1 on target cells and can be blocked by HIT8a and SK1. Clone S21010F does not interfere with the binding of anti-human CD8β antibodies S21011A or QA20A40. Clone S21010F shows comparable staining signals on 4% paraformaldehyde-fixed cells and unfixed cells.\u003cbr\u003e\nRRID: AB_3683437 (BioLegend Cat. No. 388813) AB_3683437 (BioLegend Cat. No. 388814)\u003cbr\u003e\nStructure: Ig superfamily\u003cbr\u003e\nDistribution: Subset of T Cells, most thymocytes, NK cells\u003cbr\u003e\nFunction: TCR co-receptor\u003cbr\u003e\nInteraction: CD8β\u003cbr\u003e\nLigand\/Receptor: MHC Class I molecules\u003cbr\u003e\nCell Type: NK cells, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego. Leahy, D.J, 1995. FASEB J. 9:17-25.\u003cbr\u003e\nGene ID: 925\u003cbr\u003e\nUniProt: View information about CD8alpha on UniProt.org\u003cbr\u003e\nClone: S21010F\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T8, Leu2, CD8 Antigen\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866272518313,"sku":"388814","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-388814","provider":"Iright","version":"1.0","type":"link"}