{"product_id":"biolegend-393507","title":"Biolegend, 393507, PE anti-human CD244 (2B4) Antibody, 25tests","description":"\u003cp\u003eCD244, known as 2B4, is a 38 kD type I transmembrane protein. It is a member of the CD2 subset of the immunoglobulin superfamily (IgSF) molecules. CD244 is expressed on NK cells, a subset of T cells (including most CD8 + T cells and γ\/δ T cells), monocytes, basophils, and eosinophils. CD48 is the ligand of CD244. It has been reported that ligation of human CD244 results in enhanced NK cell cytotoxicity and cytokine production. Recent studies have shown that human CD244, like murine CD244, has both activating and inhibitory functions, which are dependent on the density of surface 2B4 expression, degree of ligation, and the level of the adaptor molecule SAP expression.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human NK cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: redirected killing assays (rADCC) in NK cells1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Nakajima H, et al. 2000. Eur J Immunol. 30:3309. (Cytotoxicity, IP) Tassi I, Colonna M. 2005. J. Immunol. 7996-8002. (IP)\u003cbr\u003e\nRRID: AB_2734465 (BioLegend Cat. No. 393507) AB_2734466 (BioLegend Cat. No. 393508)\u003cbr\u003e\nStructure: 38 kD type I transmembrane protein, CD2 family member, Ig superfamily\u003cbr\u003e\nDistribution: NK, T subset, monocytes, basophils, and eosinophils\u003cbr\u003e\nFunction: Activation and inhibition of NK cell function\u003cbr\u003e\nLigand\/Receptor: CD48\u003cbr\u003e\nCell Type: Basophils, Eosinophils, Monocytes, NK cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Lee KM, et al. 2003. J. Immunol. 170:4881. Chlewicki LK, et al. 2008. J. Immunol. 180:8159. Messmer B, et al. 2006. J. Immunol. 176:4646. Boles KS, et al. 2001. Immunol. Rev. 181:234.\u003cbr\u003e\nGene ID: 51744\u003cbr\u003e\nUniProt: View information about CD244 on UniProt.org\u003cbr\u003e\nClone: 2-69\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: 2B4, NAIL, SLAMF4\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860862849193,"sku":"393507","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-393507","provider":"Iright","version":"1.0","type":"link"}