{"product_id":"biolegend-399614","title":"Biolegend, 399614, APC\/Fire™ 810 anti-human CD64 Antibody, 100tests","description":"\u003cp\u003eCD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes\/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG\/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human CD64-Fc (1257-FC-050)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC\/Fire™ 810 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * APC\/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm. Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC\/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: In-house testing suggests that S18012E partially blocks the binding of clone 10.1 but not clone S18012C. Clone 10.1 recognizes the EC3 epitope of CD64. While both CD64 subunits contain EC3 domains, clone 10.1 preferentially binds to CD64A (FcγRIA) but not CD64B (FcγRIB).\u003cbr\u003e\nRRID: AB_2904424 (BioLegend Cat. No. 399613) AB_2904424 (BioLegend Cat. No. 399614)\u003cbr\u003e\nStructure: Ig superfamily, type I glycoprotein, 72 kD\u003cbr\u003e\nDistribution: Monocytes, macrophages, dendritic cells, activated granulocytes\u003cbr\u003e\nFunction: Phagocytosis, ADCC\u003cbr\u003e\nLigand\/Receptor: IgG receptor\u003cbr\u003e\nCell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes\u003cbr\u003e\nBiology Area: Immunology, Inhibitory Molecules\u003cbr\u003e\nMolecular Family: CD Molecules, Fc Receptors\u003cbr\u003e\nAntigen References: Hulett MD \u0026amp; Hogarth PM. 1994. Adv Immunol. 57:1-127. van de Winkel JG \u0026amp; Capel PJ. 1993. Immunol Today. 14:215-21.\u003cbr\u003e\nGene ID: 2209\u003cbr\u003e\nUniProt: View information about CD64 on UniProt.org\u003cbr\u003e\nClone: S18012E\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: FcγRI, FcR I\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866245910697,"sku":"399614","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-399614","provider":"Iright","version":"1.0","type":"link"}