{"product_id":"biolegend-400211","title":"Biolegend, 400211, PE Mouse IgG2a, κ Isotype Ctrl Antibody, 25μg","description":"\u003cp\u003eThe MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat, and human tissues.\u003cbr\u003e\n25μg\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The immunoglobulin was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The immunoglobulin solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).\u003cbr\u003e\nProduct Citations: Gurses K, et al. 2014. Atherosclerosis. 237:784. PubMed Campbell KA, et al. 2023. Commun Biol. 6:264. PubMed Polisetti N, et al. 2023. Int J Mol Sci. 24:. PubMed Oh J, et al. 2023. Nat Commun. 14:3278. PubMed Grotheer V, et al. 2021. Sci Rep. 11:11968. PubMed Polisetti N, et al. 2022. Bio Protoc. 12: . PubMed Shen JZ, et al. 2022. Mol Cell. 82:1123. PubMed Vallverd J, et al. 2021. Nature Protocols. 16(5):2542-2563. PubMed Ameres S, et al. 2014. J Immunol. 192:5894. PubMed Obermayer A, et al. 2014. PLoS One. 9:97784. PubMed Fielding CA et al. 2017. eLife. 6 pii: e22206. PubMed Zhao P, et al. 2022. Cancers (Basel). 14:. PubMed Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed Olga Alekhina, Adriano Marchese 2016. J Biol Chem. 291(50):26083-26097. PubMed Yang C, et al. 2020. J Control Release. 326:324. PubMed Botha J, et al. 2021. Biomedicines. 9:. PubMed Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed Raffray L, et al. 2016. PLoS One. 11:e0165716. PubMed Blank A, et al. 2021. J Pathol. 253:160. PubMed Liu Z, et al. 2021. Molecules. 26:. PubMed McPherson HR, et al. 2021. Elife. 10:. PubMed Tang S, et al. 2021. Front Cell Dev Biol. 9:670913. PubMed Iwabuchi R, et al. 2021. Frontiers in Immunology. 12:643040. PubMed Metwally H, et al. 2020. Sci Signal. :13. PubMed Martens CR, et al. 2022. PLoS Pathog. 18:e1010342. PubMed Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed Hara M, et al. 2021. Materials (Basel). 14:. PubMed Tritschler H, et al. 2021. Cells. 10:. PubMed James KR, et al. 2020. Nat Immunol. 1.113194444. PubMed Morris AB, et al. 2020. Immunity. 52(1):136-150.e6.. PubMed Ponath V, et al. 2021. Int J Mol Sci. 22:. PubMed Okita R, et al. 2021. Thorac Cancer. 12:775. PubMed Zhang B, et al. 2021. Nat Commun. 12:1714. PubMed Holst K, et al. 2015. J Cell Sci. 128: 2866-2880. PubMed Kang YH, et al. 2019. Nat Commun. 10:912. PubMed Nevins AM, et al. 2017. Methods Mol Biol. 1722:151. PubMed Song S, et al. 2021. Front Immunol. 12:705140. PubMed Schmidt T, et al. 2015. PLoS One. 10: 0132703. PubMed Zhang HC, et al. 2018. Int J Mol Med. 41:13. PubMed Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed Samuel RM, et al. 2020. Cell Stem Cell. 27:876. PubMed Aguilar–Briseñ o JA, et al. 2020. Nat Commun. 2.664583333. PubMed\u003cbr\u003e\nRRID: AB_326460 (BioLegend Cat. No. 400211) AB_326460 (BioLegend Cat. No. 400212)\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nClone: MOPC-173\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: IgG2a gene is deleted in some mouse strains, which ones are they?\u003cbr\u003e\nA: IgG2a gene is deleted in C57Bl\/6, C57Bl\/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866136563881,"sku":"400211","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-400211","provider":"Iright","version":"1.0","type":"link"}