{"product_id":"biolegend-400634","title":"Biolegend, 400634, FITC Rat IgG2b, κ Isotype Ctrl Antibody, 100tests","description":"\u003cp\u003eThe isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.\u003cbr\u003e\n100tests\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Trinitrophenol + KLH\u003cbr\u003e\nFormulation: test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The immunoglobulin was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: test sizes: lot-specific; µg sizes: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this rat IgG2b, ? isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Cervantes-Barragan L, et al. 2007. Blood 109:1131. Zeiser R, et al. 2007. Blood 109:2225. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed Yi H, et al. 2009. Blood 113:5819. PubMed Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed Lei GS, et al. 2015. Infect Immun. 83:572. PubMed Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed\u003cbr\u003e\nProduct Citations: Elbasiony E, et al. 2023. NPJ Aging. 9:2. PubMed Pioli KT, et al. 2023. iScience. 26:106223. PubMed Dona MSI, et al. 2023. Basic Res Cardiol. 118:11. PubMed Kase N, et al. 2023. Front Cell Dev Biol. 11:1022081. PubMed Ding L, et al. 2023. J Clin Invest. 133: . PubMed Ye B, et al. 2022. Cell Mol Immunol. 19:619. PubMed Chen X, et al. 2021. Theranostics. 11:4655. PubMed Sakamoto T, et al. 2021. Commun Biol. 4:1165. PubMed Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed Tanner MA, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H633. PubMed Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed Reynolds A, et al. 2015. J Immunol. 194:231. PubMed Colliou N, et al. 2018. Gut Microbes. 9:279. PubMed Yuan F, et al. 2022. Front Immunol. 13:798583. PubMed Garg M, et al. 2021. Cell Reports. 34(6):108736. PubMed O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed Shiraki N, et al. 2022. Stem Cell Reports. 17:221. PubMed Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed Wang X, et al. 2018. Cell Death Dis. 9:578. PubMed Yang B, et al. 2022. Bioengineered. 13:2685. PubMed López MJ, et al. 2018. Invest Ophthalmol Vis Sci. 59:5671. PubMed Li J, et al. 2022. Nat Commun. 13:4032. PubMed Niemi JP, et al. 2020. Methods Mol Biol. 2143:207. PubMed Kumagai S, et al. 2020. Immunity. 53(1):187-203.e8. PubMed Wang S, et al. 2021. Parasite. 28:13. PubMed Ohgomori T et al. 2019. J Comp Neurol. 528(1):14-31 . PubMed Wang Y, et al. 2022. Front Immunol. 13:847581. PubMed Wang F, et al. 2018. Oncogene. 37:286. PubMed Grisanti LA, et al. 2019. JCI Insight. 5. PubMed Hao T, et al. 2019. Nat Commun. 10:3665. PubMed Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed Gupta P, et al. 2016. PLoS One. 11: 0154725. PubMed Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed Wu KKL, et al. 2021. Nat Commun. 12:6637. PubMed\u003cbr\u003e\nRRID: AB_893678 (BioLegend Cat. No. 400633) AB_326549 (BioLegend Cat. No. 400605) AB_893662 (BioLegend Cat. No. 400634) AB_326550 (BioLegend Cat. No. 400606)\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nClone: RTK4530\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866210980009,"sku":"400634","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-400634","provider":"Iright","version":"1.0","type":"link"}