{"product_id":"biolegend-402020","title":"Biolegend, 402020, GoInVivo™ Purified Syrian Hamster IgG Isotype Ctrl Antibody, 25mg","description":"\u003cp\u003eThe SHG-1 immunoglobulin is of Syrian hamster IgG isotype and was chosen as an isotype control after screening on variety of resting, activated, live, and fixed mouse, rat and human tissues.\u003cbr\u003e\n25mg\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Syrian Hamster\u003cbr\u003e\nImmunogen: Trinitrophenol + KLH\u003cbr\u003e\nFormulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.\u003cbr\u003e\nEndotoxin Level: Less than 1.0 EU\/mg of the protein (\u0026lt; 0.1 pg\/µg of the protein) as determined by the LAL test.\u003cbr\u003e\nPreparation: The GoInVivo™ antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg\/mL and 3 mg\/mL.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.\u003cbr\u003e\nApplication: FC - Quality tested ICFC, WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.\u003cbr\u003e\nApplication Notes: The SHG-1 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 402014) as negative control.\u003cbr\u003e\nAdditional Product Notes: GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Nishimoto H, et al. 2005. Blood. 106:4241.\u003cbr\u003e\nProduct Citations: Ishikura N, et al. 2022. Oncol Rep. 47:. PubMed\u003cbr\u003e\nRRID: AB_3097020 (BioLegend Cat. No. 402019) AB_3097020 (BioLegend Cat. No. 402020) AB_3097020 (BioLegend Cat. No. 402021) AB_3097020 (BioLegend Cat. No. 402022) AB_3097020 (BioLegend Cat. No. 402023) AB_3097020 (BioLegend Cat. No. 402024)\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nClone: SHG-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nIsotype: Syrian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866294276265,"sku":"402020","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-402020","provider":"Iright","version":"1.0","type":"link"}