{"product_id":"biolegend-405237","title":"Biolegend, 405237, Alexa Fluor® 647 Streptavidin, 100μg","description":"\u003cp\u003eStreptavidin binds to biotin with high affinity. Streptavidin-Alexa Fluor® 647 is useful for detecting biotinylated antibodies. The excitation of Alexa Fluor® 647 by 633 nm \/ 635 nm laser light induces a light emission maximum of 668 nm.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat, All Species\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: Streptavidin was conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml (concentration relates to the Streptavidin only component of the conjugate)\u003cbr\u003e\nStorage \u0026amp; Handling: The streptavidin-Alexa Fluor® 647 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested ICFC - Verified\u003cbr\u003e\nRecommended Usage: This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Alexa Fluor® 647 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Streptavidin-Alexa Fluor® 647 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Alexa Fluor® 647 is 60 kD and Streptavidin alone is 52 kD.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Chappaz S, et al. 2007. Blood doi:10.1182\/blood-2007-02-074245. Nishimoto KP,et al. 2008. J. Immunol. 181:4010.PubMed Niki T, et al. 2009. J. Biol. Chem. 284:32344. PubMed Shibui A, et al. 2011. Exp Parasitol. 129:318. PubMed Scatizzi JC, et al. 2012. J. Immunol. 188:3307. PubMed Yamakawa N, et al. 2012. Int Immunol. PubMed Shibata T, et al. 2012. Int Immunol. 24:613. PubMed Baccala R, et al. 2012. J. Immunol. 189:5976. PubMed Grevers LC, et al. 2013. Ann Reheum Dis. 72:278. PubMed Woo SJ, et al. 2013. J Leukoc Biol. 93:363. PubMed Ashbaugh JJ, et al. 2013. J. Immunol. 190:4525. PubMed Reading JL, 2013. J. immunol. 190:4542. Pubmed. Kanno A, et al. 2013. Int Immunol. 25:413. PubMed Gunaydin G, et al. 2014. Vaccine. 32:470. PubMed Shade KT, et al. 2015. J Exp Med. 212:457. PubMed\u003cbr\u003e\nProduct Citations: Janeczek A, et al. 2017. Nanomedicine. 10.2217\/nnm-2016-0386. PubMed Bednarski E, et al. 2022. MBio. 13:e0084022. PubMed Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed Deisher A, et al. 2022. STAR Protoc. 3:101663. PubMed Melzi E, et al. 2022. Immunity. 55:2168. PubMed Hu Q, et al. 2022. Cell Rep. 40:111035. PubMed Weskamm LM, et al. 2022. Cell Rep Med. 3:100685. PubMed Wang Z, et al. 2022. J Exp Med. 219: . PubMed Taft JM, et al. 2022. Cell. 185:4008. PubMed Schaefer-Babajew D, et al. 2023. Nature. 613:735. PubMed Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed Weskamm LM, et al. 2022. STAR Protoc. 3:101902. PubMed Bettini E, et al. 2022. STAR Protoc. 3:101840. PubMed Shilts J, et al. 2023. PLoS Biol. 21:e3001959. PubMed Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed Agudelo M, et al. 2021. J Exp Med. :218. PubMed Gaebler C, et al. 2021. Nature. 639:591. PubMed Pobezinskaya EL et al. 2019. Front Immunol. 1.079861111 . PubMed Schiesser JV, et al. 2021. Sci Rep. 11:8315. PubMed Agudelo M, et al. 2022. J Exp Med. 219:. PubMed Noreng S, et al. 2022. Nat Commun. 13:6079. PubMed Shade K, et al. 2015. J Exp Med. 212:457. PubMed Biram A, et al. 2020. Cell Rep. 30:1910. PubMed Needham BD, et al. 2022. Nature. 602:647. PubMed Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed Zhou D, et al. 2022. Nat Commun. 13:5174. PubMed Zhang R, et al. 2021. MAbs. 13:1904546. PubMed Wang Z, et al. 2021. bioRxiv. . PubMed Glaros V, et al. 2021. Immunity. 54:2005. PubMed Yap K, et al. 2022. STAR Protoc. 3:101139. PubMed Cho A, et al. 2021. Nature. Online ahead of print. PubMed Charles TP, et al. 2021. PLoS Pathog. 17:e1009257. PubMed Van Den Berge N, et al. 2019. Acta Neuropathol. 138(4):535. PubMed Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed Alameh MG, et al. 2021. Immunity. 54:2877. PubMed Gilpin TE, et al. 2021. J Immunol. 207:1065. PubMed Van Simaeys D, et al. 2022. Nat Commun. 13:1815. PubMed Biram A, et al. 2020. Bio Protoc. e3562:10. PubMed Lee M, et al. 2020. Elife. :9. PubMed Duong BTV, et al. 2020. EBioMedicine. 61:103031. PubMed Wang X, et al. 2021. EMBO J. 40:e105926. PubMed Kulkarni N, et al. 2017. Eur J Appl Physiol.. 10.1007\/s00421-017-3704-z. PubMed Wang Z, et al. 2021. Nature. 592:616. PubMed Puentes-Mestril C, et al. 2021. J Neurosci. 41:5386. PubMed Wang Z, et al. 2022. J Exp Med. 219:. PubMed Wang Z, et al. 2020. Elife. 9:00. PubMed Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed Wang Z, et al. 2021. Nature. 595:426. PubMed Lederer K, et al. 2022. Cell. . PubMed Gaebler C, et al. 2020. bioRxiv. . PubMed Plewes MR, et al. 2020. FASEB J. 5299:34. PubMed Chmielewski M and Abken H 2017. Cell Rep.. 10.1016\/j.celrep.2017.11.063. PubMed Frohlich J, et al. 2020. Aging (Albany NY). 12:20024. PubMed Pabbisetty S, et al. 2016. Proc Natl Acad Sci U S A. 113: E4662 - E4670. PubMed Yoshikawa S, et al. 2016. Sci Rep. 6:18738. PubMed Tsai S, et al. 2018. Cell Metab. 28:922. PubMed Cho A, et al. 2022. J Exp Med. 219:. PubMed\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about Biotin on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Streptavidin-Alexa Fluor® 647, Streptavidin-AF647, SAV\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866503532713,"sku":"405237","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-405237","provider":"Iright","version":"1.0","type":"link"}