{"product_id":"biolegend-405358","title":"Biolegend, 405358, Spark Violet™ 538 Streptavidin, 100μg","description":"\u003cp\u003eStreptavidin is a 52.8 kDa tetrameric protein obtained from Streptomyces avidinii . It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10 -14 mol\/L. It is used to detect biotinylated proteins in a wide range of applications.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat, All Species\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: Streptavidin is conjugated with Spark Violet™ 538 under optimal conditions\u003cbr\u003e\nConcentration: 0.5 mg\/mL (concentration relates to the Streptavidin only component of the conjugate)\u003cbr\u003e\nStorage \u0026amp; Handling: The streptavidin-Spark Violet™ 538 solution should be stored undiluted between 2°C and 8°C, and protected from exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this Streptavidin-Spark Violet™ 538 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Spark Violet™ 538 conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.5 µg per 106 cells in 100 µL staining volume. It is recommended that the reagent be titrated for optimal performance for each application. * Spark Violet™ 538 has a maximum excitation of 396 nm and a maximum emission of 545 nm.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Streptavidin-Spark Violet™ 538 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Spark Violet™ 538 is 60 kD and Streptavidin alone is 52 kD.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about Biotin on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Streptavidin-Spark Violet™, SAv-Spark Violet™ 538, streptavidin Spark Violet™ 538\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866510020777,"sku":"405358","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-405358","provider":"Iright","version":"1.0","type":"link"}